Surface modification of porous polycaprolactone/biphasic calcium phosphate scaffolds for bone regeneration in rat calvaria defect
- Authors
- Kim, Ji-Hyun; Linh, Nguyen T. B.; Min, Young K.; Lee, Byong-Taek
- Issue Date
- Oct-2014
- Publisher
- SAGE Publications
- Keywords
- Porous PCL; BCP; aminolysis; surface modification; collagen immobilization; bone regeneration
- Citation
- Journal of Biomaterials Applications, v.29, no.4, pp 624 - 635
- Pages
- 12
- Journal Title
- Journal of Biomaterials Applications
- Volume
- 29
- Number
- 4
- Start Page
- 624
- End Page
- 635
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/11836
- DOI
- 10.1177/0885328214539822
- ISSN
- 0885-3282
1530-8022
- Abstract
- In this study, polycaprolactone scaffolds fabricated by a salt-leaching process were loaded with biphasic calcium phosphate successfully to improve the osteoconductivity in bone regeneration. The surface of polycaprolactone/biphasic calcium phosphate scaffolds was aminolyzed by 1,6-hexamethylenediamine to introduce amino groups onto the surface, which was verified qualitatively by ninhyrin staining. Collagen was further immobilized on the aminolyzed porous polycaprolactone via N-ethyl-N-(3-dimethylaminopropy) carbodiimide hydrochloride/hydroxy-2,5-dioxopyrolidine-3-sulfonic acid sodium cross-linking. The pore size of polycaprolactone/biphasic calcium phosphate-collagen scaffolds was 200-300 mu m, which was suitable for bone in-growth. The X-ray photoelectron spectroscopy confirmed the coupling of collagen immobilized on the surface of polycaprolactone/biphasic calcium phosphate. In vitro results demonstrated that the spreading and viability of MC3T3-E1 cells were remarkably improved in the polycaprolactone/biphasic calcium phosphate-collagen scaffolds. The in vivo study was carried out by implanting the porous polycaprolactone, polycaprolactone/biphasic calcium phosphate, and polycaprolactone/biphasic calcium phosphate-collagen to the skulls of rats. Although the addition of biphasic calcium phosphate particles in the polycaprolactone scaffolds does not have a strong effect on the new bone formation, the immobilization of collagen on the polycaprolactone/biphasic calcium phosphate scaffolds significantly improved the bone regeneration even though the implantation time was short, 6 weeks. The present results provide more evidence that functionalizing polycaprolactone with biphasic calcium phosphate and collagen may be a feasible way to improve the osteoconduction in bone regeneration.
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Collections - College of Medicine > Department of Regenerative Medicine > 1. Journal Articles
- College of Medicine > Department of Physiology > 1. Journal Articles
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