Cross Talk with Hematopoietic Cells Regulates the Endothelial Progenitor Cell Differentiation of CD34 Positive Cells
- Authors
- Kwon, Sang-Mo; Lee, Jun-Hee; Lee, Sang-Hun; Jung, Seok-Yun; Kim, Da-Yeon; Kang, Song-Hwa; Yoo, So-Young; Hong, Jong-Kyu; Park, Ji-Hye; Kim, Jung-Hee; Kim, Sung-Wook; Kim, Yeon-Ju; Lee, Sun-Jin; Kim, Hwi-Gon; Asahara, Takayuki
- Issue Date
- 28-Aug-2014
- Publisher
- Public Library of Science
- Keywords
- Cross Talk with Hematopoietic Cells Regulates the Endothelial Progenitor Cell Differentiation of CD34 Positive Cells
- Citation
- PLoS ONE, v.9, no.8
- Journal Title
- PLoS ONE
- Volume
- 9
- Number
- 8
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/11945
- DOI
- 10.1371/journal.pone.0106310
- ISSN
- 1932-6203
- Abstract
- Introduction: Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear. Methods: In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34(+) and CD34(-) cells, and determined the optimal concentrations of CD34(+) cells and CD34(-) cells for spindle-shaped EPC differentiation. Results: Functionally, the coculture of CD34(+) and CD34(-) cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34(+) cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34(+) and CD34(-) cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3(+)) markedly accelerated the early EPC status of CD34(+) cells, while macrophages (CD11b(+)) or megakaryocytes (CD41(+)) specifically promoted large EPC colonies. Conclusion: Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34(+) cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy.
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Collections - College of Medicine > Department of Biochemistry > 1. Journal Articles
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