고도 정제된 Hyaluronidase의 랫드에 대한아나필락시스 쇼크 반응시험

Abstract

The hyaluronidases are a family of enzymes that degrade hyaluronic acid. In humans, there are six associated genes, including HYAL1, HYAL2, HYAL3, and PH-20/SPAM1. Ovine testicular hyaluronidase (OTH) has been used in several medical fields for many years. This study was performed to evaluate the antigenicity of highly purified hyaluronidase (p-HAase) from ovine testis using an active systemic anaphylaxis (ASA) test in rats. In ASA test using rats, there were no significant clinical symptoms in addition to licking nose in all individuals of low (150 IU/kg) and high (1,500 IU/kg) dose of both groups treated with only p-HAase and co-treated with p-HAase and Freund's complete adjuvant (FCA) group. In conclusion, p-HAase, as showed in ASA test, did not cause anaphylatic shock when treated 6 and 60 times higher than clinically intended dose, nor induce IgE, so that might not have antigenic properties in rats....