고도 정제된 Hyaluronidase의 랫드에 대한아나필락시스 쇼크 반응시험
- Authors
- 한만덕
- Issue Date
- Jun-2014
- Publisher
- 순천향대학교 기초과학연구소
- Keywords
- Hyaluronidase; Hyaluronic acid; Ovine testis; Antigenicity; Active systemicanaphylaxis (ASA)
- Citation
- 순천향 자연과학연구 논문집, v.20, no.1, pp.55 - 61
- Journal Title
- 순천향 자연과학연구 논문집
- Volume
- 20
- Number
- 1
- Start Page
- 55
- End Page
- 61
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/12105
- ISSN
- 1738-4761
- Abstract
- The hyaluronidases are a family of enzymes that degrade hyaluronic acid. In humans, there are six associated genes, including HYAL1, HYAL2, HYAL3, and PH-20/SPAM1. Ovine testicular
hyaluronidase (OTH) has been used in several medical fields for many years. This study was performed to evaluate the antigenicity of highly purified hyaluronidase (p-HAase) from ovine
testis using an active systemic anaphylaxis (ASA) test in rats. In ASA test using rats, there were no significant clinical symptoms in addition to licking nose in all individuals of low (150
IU/kg) and high (1,500 IU/kg) dose of both groups treated with only p-HAase and co-treated with p-HAase and Freund's complete adjuvant (FCA) group. In conclusion, p-HAase, as showed
in ASA test, did not cause anaphylatic shock when treated 6 and 60 times higher than clinically intended dose, nor induce IgE, so that might not have antigenic properties in rats....
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Collections - College of Natural Sciences > Department of Life Science and Biotechnology > 1. Journal Articles
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