Comparison Analysis of Immune Cells between CT26 Tumor Bearing Mice and Normal Mice

Abstract

It has well studied that immune cells are strongly related to tumor progression and tumor suppression. To identify thedifference of immune cell between tumor bearing mice and normal mice, we examined systemically the immune cell ofCT26 tumor bearing mice on 21 days after tumor cell administration. As previously reported, CD4+ and CD8+ T cellspopulation of tumor bearing mice significantly decreased 38% and 30% on day 21 compared to that of normal mice,respectively. All subpopulation of CD4 and CD8+ T cell significantly decreased, except CD49b+ T cell subpopulation. But, myeloid cell population (CD11bhigh and all Gr-1+ subpopulation) of tumor bearing mice significantly increased onday 21. Especially, all subpopulation of CD11b+Gr-1+ cell of tumor bearing mice significantly increased on day 21. Also, Foxp3+CD25high CD4 T cell (regulatory T cells) population significantly increased on day 21. These resultssuggest that tumor can induce the decline of T lymphocyte and the expansion of myeloid cells and regulatory T cells,and provide the basic information for the study of tumor immunology.