Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometryProteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry
- Other Titles
- Proteomic analysis of domestic pig pancreas during development using two-dimensional electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry
- Authors
- 안지예; 김일용; 오새진; 황혜숙; 이순신; 김요나; 신재훈; 윤여성; 성제경
- Issue Date
- 2014
- Publisher
- 한국실험동물학회
- Keywords
- Domestic pig; pancreas; development; 2-DE proteomics; MALDI-TOF
- Citation
- Laboratory Animal Research, v.30, no.2, pp 45 - 53
- Pages
- 9
- Journal Title
- Laboratory Animal Research
- Volume
- 30
- Number
- 2
- Start Page
- 45
- End Page
- 53
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/12868
- ISSN
- 1738-6055
2233-7660
- Abstract
- Pig pancreas may be a therapeutic resource for human diabetic patients. However, this potential ishindered by a lack of knowledge of the molecular events of pig pancreas development. In this study, theembryonic day 60, neonate and 6-month protein profiles of pig pancreas were ascertained at using twodimensionalgel electrophoresis and matrix assisted laser desorption/ionization-time of flight massspectrometry. Twenty four proteins were differentially expressed during pig pancreas development. Among them, 12 spots increased and 7 spots decreased according to development. The expression of 5protein were highest at birth. Expression of digestive enzymes including trypsin, pancreatic triacylglycerollipase and pancreatic alpha-amylase was elevated in adults, whereas chymotrypsins were highly expressedin neonates. Proteins that were abundantly expressed during gestation were alpha-1-antitrypsin, alphafetoproteinand transferrins. Taken together, we found out that several proteins were significantly up- ordown- regulated from pig pancreas based on developmental stage. This study will provide basis forunderstanding development of pig pancreas.
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Collections - College of Medical Sciences > Department of Biomedical Laboratory Science > 1. Journal Articles
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