Fenobam promoted the neuroprotective effect of PEP-1-FK506BP following oxidative stress by increasing its transduction efficiency
- Authors
- Ahn, Eun Hee; Kim, Dae Won; Shin, Min Jea; Jo, Hyo Sang; Eom, Seon Ae; Kim, Duk-Soo; Park, Eun Young; Park, Jong Hoon; Cho, Sung-Woo; Park, Jinseu; Eum, Won Sik; Son, Ora; Hwang, Hyun Sook; Choi, Soo Young
- Issue Date
- 30-Nov-2013
- Publisher
- 생화학분자생물학회
- Keywords
- Fenobam; Ischemic damage; Neuroprotection; PEP-1-FK506BP; Protein transduction domains
- Citation
- BMB Reports, v.46, no.11, pp 561 - 566
- Pages
- 6
- Journal Title
- BMB Reports
- Volume
- 46
- Number
- 11
- Start Page
- 561
- End Page
- 566
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13202
- DOI
- 10.5483/BMBRep.2013.46.11.080
- ISSN
- 1976-6696
1976-670X
- Abstract
- We examined the ways in which fenobam could promote not only the transduction of PEP-1-FK506BP into cells and tissues but also the neuroprotective effect of PEP-1-FK506BP against ischemic damage. Fenobam strongly enhanced the protective effect of PEP-1-FK506BP against H2O2-induced toxicity and DNA fragmentation in C6 cells. In addition, combinational treatment of fenobam with PEP-1-FK506BP significantly inhibited the activation of Akt and MAPK induced by H2O2, compared to treatment with PEP-1-FK506BP alone. Interestingly, our results showed that fenobam significantly increased the transduction of PEP-1-FK506BP into both C6 cells and the hippocampus of gerbil brains. Subsequently, a transient ischemic gerbil model study demonstrated that fenobam pretreatment led to the increased neuroprotection of PEP-1-FK506BP in the CA1 region of the hippocampus. Therefore, these results suggest that fenobam can be a useful agent to enhance the transduction of therapeutic PEP-1-fusion proteins into cells and tissues, thereby promoting their neuroprotective effects.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Anatomy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13202)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.