Cloning, Characterization and Effect of TmPGRP-LE Gene Silencing on Survival of Tenebrio Molitor against Listeria monocytogenes Infection
- Authors
- Tindwa, Hamisi; Patnaik, Bharat Bhusan; Kim, Dong Hyun; Mun, Seulgi; Jo, Yong Hun; Lee, Bok Luel; Lee, Yong Seok; Kim, Nam Jung; Han, Yeon Soo
- Issue Date
- Nov-2013
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- peptidoglycan recognition protein; Tenebrio molitor; Listeria monocytogenes; PGRP domain; RNA interference
- Citation
- International Journal of Molecular Sciences, v.14, no.11, pp 22462 - 22482
- Pages
- 21
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 14
- Number
- 11
- Start Page
- 22462
- End Page
- 22482
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13275
- DOI
- 10.3390/ijms141122462
- ISSN
- 1661-6596
1422-0067
- Abstract
- Peptidoglycan recognition proteins (PGRPs) are a family of innate immune molecules that recognize bacterial peptidoglycan. PGRP-LE, a member of the PGRP family, selectively binds to diaminopimelic acid (DAP)-type peptidoglycan to activate both the immune deficiency (Imd) and proPhenoloxidase (proPO) pathways in insects. A PGRP-LE-dependent induction of autophagy to control Listeria monocytogenes has also been reported. We identified and partially characterized a novel PGRP-LE homologue, from Tenebrio molitor and analyzed its functional role in the survival of the insect against infection by a DAP-type PGN containing intracellular pathogen, L. monocytogenes. The cDNA is comprised of an open reading frame (ORF) of 990 bp and encodes a polypeptide of 329 residues. TmPGRP-LE contains one PGRP domain, but lacks critical residues for amidase activity. Quantitative RT-PCR analysis showed a broad constitutive expression of the transcript at various stages of development spanning from larva to adult. RNAi mediated knockdown of the transcripts, followed by a challenge with L. monocytogenes, showed a significant reduction in survival rate of the larvae, suggesting a putative role of TmPGRP-LE in sensing and control of L. monocytogenes infection in T. molitor. These results implicate PGRP-LE as a defense protein necessary for survival of T. molitor against infection by L. monocytogenes.
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