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Effect of acrolein, a hazardous air pollutant in smoke, on human middle ear epithelial cells

Authors
Song, Jae-JunLee, Jong DaeLee, Byung DonChae, Sung WonPark, Moo Kyun
Issue Date
Oct-2013
Publisher
Elsevier BV
Keywords
Acrolein; Human middle ear epithelial cells; Otitis media; Middle ear
Citation
International Journal of Pediatric Otorhinolaryngology, v.77, no.10, pp 1659 - 1664
Pages
6
Journal Title
International Journal of Pediatric Otorhinolaryngology
Volume
77
Number
10
Start Page
1659
End Page
1664
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13343
DOI
10.1016/j.ijporl.2013.07.021
ISSN
0165-5876
1872-8464
Abstract
Objective: Acrolein is a hazardous air pollutant. Tobacco smoke and indoor air pollution are the main causes of human exposure. Acrolein has been shown to cause cytotoxicity in the airways and induce inflammation and mucin production in pulmonary cells. We investigated whether acrolein caused cytotoxicity, induced inflammation or increased expression of mucin in immortalized human middle ear epithelial cell lines (HMEECs). Methods: Cytotoxicity following acrolein treatment was investigated using the MTT assay, flow cytometry, and Hoechst 33342 staining of HMEECs. We measured expression of inflammatory cytokines tumor necrosis factor (TNF)-alpha and cyclo-oxygenase (COX)-2 and the mucin gene MUC5AC using semi-quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting. Results: Exposure to >50 mu g/mL acrolein caused a decrease in cell viability. Acrolein induced apoptosis and necrosis at 50 mu g/mL. Acrolein at 5-50 mu g/mL increased expression of TNF-alpha and COX-2, as shown by RT-PCR and Western blotting. Acrolein exposure at 5-50 mu g/mL for 2-24 h increased MUC5AC expression, as determined by RT-PCR. Conclusion: Acrolein decreased cell viability, induced an inflammatory response, and increased mucin gene expression in HMEECs. These findings support the hypothesis that acrolein, a hazardous air pollutant in tobacco smoke and ambient air, is a risk factor for otitis media. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
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