Hepatoprotective effects of reynosin against thioacetamide-induced apoptosis in primary hepatocytes and mouse liver
- Authors
- Lim, Soohyun; Lee, Sung-Jin; Nam, Kung-Woo; Kim, Kyeong Ho; Mar, Woongchon
- Issue Date
- Apr-2013
- Publisher
- 대한약학회
- Keywords
- Reynosin; Thioacetamide (TAA); Hepatocyte; Apoptosis
- Citation
- Archives of Pharmacal Research, v.36, no.4, pp 485 - 494
- Pages
- 10
- Journal Title
- Archives of Pharmacal Research
- Volume
- 36
- Number
- 4
- Start Page
- 485
- End Page
- 494
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/13820
- DOI
- 10.1007/s12272-013-0039-0
- ISSN
- 0253-6269
1976-3786
- Abstract
- The aim of this study was to identify the hepatoprotective effects of reynosin, sesquiterpenes from the leaves of Laurus nobilis, against thioacetamide (TAA)-induced apoptosis in primary hepatocyte cultures and an in vivo mouse model. Rat hepatocytes were isolated and pretreated with 0.13, 0.64, or 3.22 mu M reynosin and then exposed to 100 mM TAA. Reynosin treatment significantly inhibited TAA-induced apoptosis and hepatocellular DNA damage in primary rat hepatocytes. We observed an increase in levels of antiapoptotic Bcl-2, Bcl-XL mRNA and a decrease in levels of proapoptotic Bax mRNA following reynosin treatment of hepatocytes. Apoptosis in BALB/c mice was induced with intra-peritoneal injection of 200 mg/kg TAA for 2 weeks every other day. Then reynosin (5 mg/kg) and TAA were intragastrically given for 3 weeks every other day. Aspartate aminotransferase and alanine aminotransferase levels in the blood of mice were decreased in the reynosin administration group. Bcl-2 and Bcl-XL mRNA levels were increased, and the Bax mRNA level was decreased in reynosin-treated mice. Thus, reynosin inhibited TAA-induced apoptosis in primary hepatocytes and an in vivo mouse model.
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Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
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