Functional analyses of individual mating-type transcripts at MAT loci in Fusarium graminearum and Fusarium asiaticum
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hee-Kyoung | - |
dc.contributor.author | Cho, Eun Ji | - |
dc.contributor.author | Lee, Seunghoon | - |
dc.contributor.author | Lee, Young-Sang | - |
dc.contributor.author | Yun, Sung-Hwan | - |
dc.date.accessioned | 2021-08-12T02:26:06Z | - |
dc.date.available | 2021-08-12T02:26:06Z | - |
dc.date.issued | 2012-12 | - |
dc.identifier.issn | 0378-1097 | - |
dc.identifier.issn | 1574-6968 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14661 | - |
dc.description.abstract | Members of the Fusarium graminearum species (Fg) complex, which are homothallic ascomycetous species, carry two opposite mating-type (MAT) loci in a single nucleus for controlling sexual development. We investigated the roles of three (MAT1-1-1, MAT1-1-2, and MAT1-1-3) and two (MAT1-2-1 and MAT1-2-3) transcripts located at both loci in representative Fg complex species (F. graminearum and Fusarium asiaticum). In self-fertile F. graminearum strains, the transcript levels of MAT1-1-1, MAT1-2-1, and MAT1-2-3 peaked 2 days after sexual induction (dai) and then remained high until 12 dai, whereas MAT1-1-2 and MAT1-1-3 transcripts reached peak levels between 4 and 8 dai. In contrast, all of the MAT transcripts in self-sterile F. asiaticum strains accumulated at much lower levels than those in F. graminearum during the entire time. Targeted gene deletions confirmed that MAT1-1-1, MAT1-1-2, MAT1-1-3, and MAT1-2-1 were essential for self-fertility in F. graminearum, but MAT1-2-3 was not. All MAT-deleted strains (except Delta MAT1-2-3) produced recombinant perithecia when outcrossed to a self-fertile strain. These results indicate that developmental up-regulation of the individual MAT genes in both a proper fashion and quantity is critical for sexual development, and that alterations in the gene expression could be attributed to the variation in self-sterility among the Fg complex. | - |
dc.format.extent | 8 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Blackwell | - |
dc.title | Functional analyses of individual mating-type transcripts at MAT loci in Fusarium graminearum and Fusarium asiaticum | - |
dc.type | Article | - |
dc.publisher.location | 영국 | - |
dc.identifier.doi | 10.1111/1574-6968.12012 | - |
dc.identifier.scopusid | 2-s2.0-84870352529 | - |
dc.identifier.wosid | 000312081600002 | - |
dc.identifier.bibliographicCitation | FEMS Microbiology Letters, v.337, no.2, pp 89 - 96 | - |
dc.citation.title | FEMS Microbiology Letters | - |
dc.citation.volume | 337 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 89 | - |
dc.citation.endPage | 96 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | HEAD BLIGHT PATHOGEN | - |
dc.subject.keywordPlus | PERITHECIUM DEVELOPMENT | - |
dc.subject.keywordPlus | SEXUAL REPRODUCTION | - |
dc.subject.keywordPlus | SPECIES COMPLEX | - |
dc.subject.keywordPlus | GENES | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | WHEAT | - |
dc.subject.keywordPlus | INSIGHTS | - |
dc.subject.keywordAuthor | Fusarium graminearum | - |
dc.subject.keywordAuthor | mating-type genes | - |
dc.subject.keywordAuthor | self-fertility | - |
dc.subject.keywordAuthor | sexual reproduction | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
(31538) 22, Soonchunhyang-ro, Asan-si, Chungcheongnam-do, Republic of Korea+82-41-530-1114
COPYRIGHT 2021 by SOONCHUNHYANG UNIVERSITY ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.