Functional analyses of individual mating-type transcripts at MAT loci in Fusarium graminearum and Fusarium asiaticum
- Authors
- Kim, Hee-Kyoung; Cho, Eun Ji; Lee, Seunghoon; Lee, Young-Sang; Yun, Sung-Hwan
- Issue Date
- Dec-2012
- Publisher
- Blackwell
- Keywords
- Fusarium graminearum; mating-type genes; self-fertility; sexual reproduction
- Citation
- FEMS Microbiology Letters, v.337, no.2, pp 89 - 96
- Pages
- 8
- Journal Title
- FEMS Microbiology Letters
- Volume
- 337
- Number
- 2
- Start Page
- 89
- End Page
- 96
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14661
- DOI
- 10.1111/1574-6968.12012
- ISSN
- 0378-1097
1574-6968
- Abstract
- Members of the Fusarium graminearum species (Fg) complex, which are homothallic ascomycetous species, carry two opposite mating-type (MAT) loci in a single nucleus for controlling sexual development. We investigated the roles of three (MAT1-1-1, MAT1-1-2, and MAT1-1-3) and two (MAT1-2-1 and MAT1-2-3) transcripts located at both loci in representative Fg complex species (F. graminearum and Fusarium asiaticum). In self-fertile F. graminearum strains, the transcript levels of MAT1-1-1, MAT1-2-1, and MAT1-2-3 peaked 2 days after sexual induction (dai) and then remained high until 12 dai, whereas MAT1-1-2 and MAT1-1-3 transcripts reached peak levels between 4 and 8 dai. In contrast, all of the MAT transcripts in self-sterile F. asiaticum strains accumulated at much lower levels than those in F. graminearum during the entire time. Targeted gene deletions confirmed that MAT1-1-1, MAT1-1-2, MAT1-1-3, and MAT1-2-1 were essential for self-fertility in F. graminearum, but MAT1-2-3 was not. All MAT-deleted strains (except Delta MAT1-2-3) produced recombinant perithecia when outcrossed to a self-fertile strain. These results indicate that developmental up-regulation of the individual MAT genes in both a proper fashion and quantity is critical for sexual development, and that alterations in the gene expression could be attributed to the variation in self-sterility among the Fg complex.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
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