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Heat shock protein 90 inhibitor attenuates renal fibrosis through degradation of transforming growth factor-beta type II receptor

Authors
Noh, HyunjinKim, Hyun J.Yu, Mi R.Kim, Wan-YoungKim, JinRyu, Jung H.Kwon, Soon H.Jeon, Jin S.Han, Dong C.Ziyadeh, Fuad
Issue Date
Nov-2012
Publisher
Nature Publishing Group
Keywords
의약학
Citation
Laboratory Investigation, v.92, no.11, pp 1583 - 1596
Pages
14
Journal Title
Laboratory Investigation
Volume
92
Number
11
Start Page
1583
End Page
1596
URI
https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14728
DOI
10.1038/labinvest.2012.127
ISSN
0023-6837
1530-0307
Abstract
The accumulation of extracellular matrix proteins in the interstitial area is the final common feature of chronic kidney diseases. Accumulating evidence suggests that transforming growth factor (TGF)-beta 1 promotes the development of renal fibrosis. Heat shock protein (Hsp) 90 inhibitors have been shown to repress TGF-beta 1 signaling, but whether they inhibit renal fibrosis is unknown. The purpose of this study is to determine the therapeutic efficacy of Hsp90 inhibitor on renal fibrosis. In TGF-beta 1-treated HK2 cells and unilateral ureteral obstruction (UUO) kidneys, we found that 17-allylamino-17-demethoxygeldanamycin (17AAG), an Hsp90 inhibitor, decreased the expression of alpha-smooth muscle actin, fibronectin, and collagen I and largely restored the expression of E-cadherin. 17AAG inhibited TGF-beta 1-mediated phosphorylation of Smad2, Akt, glycogen synthase kinase-3 beta, and extracellular signal-regulated kinase in HK2 cells. Inhibition of Hsp90 also blocked TGF-beta 1-mediated induction of snail1. This 17AAG-induced reduction was completely restored by simultaneous treatment with proteasome inhibitor MG132. Furthermore, 17AAG blocked the interaction between Hsp90 and TGF-beta type II receptor (T beta RII) and promoted ubiquitination of T beta RII, leading to the decreased availability of T beta RII. Smurf2-specific siRNA reversed the ability of 17AAG to inhibit TGF-beta 1 signaling. The effect of 17AAG on T beta RII expression and renal fibrosis was confirmed in UUO kidneys. These findings suggest that Hsp90 inhibitor prevents the development of renal fibrosis via a mechanism dependent on Smurf2-mediated degradation of T beta R11. Laboratory Investigation (2012) 92, 1583-1596; doi:10.1038/labinvest.2012.127; published online 10 September 2012
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