Induction of Antifouling Diterpene Production by Streptomyces cinnabarinus PK209 in Co-Culture with Marine-Derived Alteromonas sp KNS-16
- Authors
- Cho, Ji Young; Kim, Myoung Sug
- Issue Date
- Oct-2012
- Publisher
- Japan Society for Bioscience Biotechnology and Agrochemistry/Nippon Nogeikagaku Kai
- Keywords
- antifouling; Streptomyces cinnabarinus PK209; lobocompactol; co-culture; Alteromonas sp KNS-16
- Citation
- Bioscience, Biotechnology and Biochemistry, v.76, no.10, pp 1849 - 1854
- Pages
- 6
- Journal Title
- Bioscience, Biotechnology and Biochemistry
- Volume
- 76
- Number
- 10
- Start Page
- 1849
- End Page
- 1854
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14786
- DOI
- 10.1271/bbb.120221
- ISSN
- 0916-8451
1347-6947
- Abstract
- An active antifouling diterpene was isolated from marine actinomycete strain PK209 and productivity was induced in a co-culture experiment. The active constituent was identified as the diterpene lobocompactol by interpretion of nuclear magnetic resonance and mass spectroscopy data. A PK209 co-culture was designed and a lobocompactol-resistant bacterium, KNS-16, was selected as co-culture competitor to induce lobocompactol production. Adding a small volume of 16-h-old KNS-16 culture to the 96-h-old PK209 culture caused rapid induction of lobocompactol production. The final yield was 2.7 mg/L, 10.4-fold higher than that collected from a single PK209 culture. The two bacteria, strains PK209 and KNS-16, were identified as Streptomyces cinnabarinus and Alteromonas sp. based on 16S rDNA sequencing. Lobocompactol showed significant antifouling activity, of 0.18 and 0.43 mu g/mL, for EC50 against the macroalga Ulva pertusa and the diatom Navicula annexa respectively. It showed activity with MIC of 61-112 mu g/mL against fouling bacteria.
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