PEP-1-metallothionein-III protein ameliorates the oxidative stress-induced neuronal cell death and brain ischemic insults
- Authors
- Sohn, Eun Jeong; Kim, Dae Won; Kim, Mi Jin; Jeong, Hoon Jae; Shin, Min Jea; Ahn, Eun Hee; Kwon, Soon Won; Kim, Young Nam; Kim, Duk-Soo; Han, Kyu Hyung; Park, Jinseu; Hwang, Hyun Sook; Eum, Won Sik; Choi, Soo Young
- Issue Date
- Oct-2012
- Publisher
- Elsevier BV
- Keywords
- Antioxidant; PEP-1-MT-III; Protein transduction; Cell viability; Ischemia; ROS
- Citation
- Biochimica et Biophysica Acta - General Subjects, v.1820, no.10, pp 1647 - 1655
- Pages
- 9
- Journal Title
- Biochimica et Biophysica Acta - General Subjects
- Volume
- 1820
- Number
- 10
- Start Page
- 1647
- End Page
- 1655
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14828
- DOI
- 10.1016/j.bbagen.2012.06.012
- ISSN
- 0304-4165
1872-8006
- Abstract
- Background: Oxidative stress is considered to be involved in a number of human diseases including ischemia. Metallothioneins (MT)-III can protect neuronal cells from the cytotoxicity of reactive oxygen species (ROS). However, MT-III proteins biological function is unclear in ischemia. Thus, we examined the protective effects of MT-III proteins on oxidative stress-induced neuronal cell death and brain ischemic insult. Methods: A human MT-III gene was fused with a protein transduction domain, PEP-1 peptide, to construct a cell permeable PEP-1-MT-III protein. PEP-1-MT-III protein was purified using affinity chromatograph. Transduced PEP-1-MT-III proteins were detected by Western blotting and immunoflourescence. Cell viability and DNA fragmentation were analyzed by 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheyltetrazolium bromide (MTT) assay and terminal dexoynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining, respectively. Brain ischemic injury was detected with immunohistochemistry. Results: Purified PEP-1-MT-III proteins transduced into astrocytes in a time- and dose-dependent manner and protected against oxidative stress-induced cell death. Also, transduced PEP-1-MT-III proteins efficiently protected cells against DNA fragmentation. Furthermore, immunohistochemical analysis revealed that PEP-1-MT-III prevented neuronal cell death in the CA1 region of the hippocampus induced by transient forebrain ischemia. We demonstrated that transduced PEP-1-MT-III protein protects against oxidative stress induced cell death in vitro and in vivo. General significance: Transduced PEP-1-MT-III protein has neuroprotective roles as an antioxidant in vitro and in vivo. PEP-1-MT-III protein is a potential therapeutic agent for various human brain diseases such as stroke. Alzheimer's disease, and Parkinson's disease. (c) 2012 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Anatomy > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14828)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.