Differential protein expression associated with heat stress in Antarctic microalga
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Kwang Man | - |
dc.contributor.author | Lee, Mi Young | - |
dc.date.accessioned | 2021-08-12T02:45:33Z | - |
dc.date.available | 2021-08-12T02:45:33Z | - |
dc.date.issued | 2012-09-20 | - |
dc.identifier.issn | 1976-0280 | - |
dc.identifier.issn | 2092-7843 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14834 | - |
dc.description.abstract | We used proteomic approaches to study the survival and defense mechanisms of Antarctic microalga under heat stress. The microalga was cultured for optimal growth at 4 degrees C, the temperature was increased to 15 degrees C, and the resultant differentially expressed proteins induced by heat stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis. Orthologues of 22 proteins showed more than two-fold changes in abundance; of these, 8 proteins were up-regulated, and 14 were down-regulated. In addition, changes in the enzyme activities and isozyme profiles of catalase, aldehyde dehydrogenase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase were investigated using an in-gel activity-staining method. Alterations in protein expression and antioxidant enzyme activity in Antarctic algae may be related to survival and defense mechanisms against elevated temperatures. | - |
dc.format.extent | 9 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | 한국바이오칩학회 | - |
dc.title | Differential protein expression associated with heat stress in Antarctic microalga | - |
dc.type | Article | - |
dc.publisher.location | 대한민국 | - |
dc.identifier.doi | 10.1007/s13206-012-6310-5 | - |
dc.identifier.scopusid | 2-s2.0-84867006299 | - |
dc.identifier.wosid | 000308779800010 | - |
dc.identifier.bibliographicCitation | BioChip Journal, v.6, no.3, pp 271 - 279 | - |
dc.citation.title | BioChip Journal | - |
dc.citation.volume | 6 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 271 | - |
dc.citation.endPage | 279 | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART001696103 | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kciCandi | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Nanoscience & Nanotechnology | - |
dc.subject.keywordPlus | NIMA-FAMILY KINASE | - |
dc.subject.keywordPlus | ANTIOXIDANT ENZYMES | - |
dc.subject.keywordPlus | CHLAMYDOMONAS-REINHARDTII | - |
dc.subject.keywordPlus | PROTEOMIC ANALYSIS | - |
dc.subject.keywordPlus | MOLECULAR-CLONING | - |
dc.subject.keywordPlus | GENE | - |
dc.subject.keywordPlus | CADMIUM | - |
dc.subject.keywordPlus | HEAT-SHOCK-PROTEIN-70 | - |
dc.subject.keywordPlus | TEMPERATURE | - |
dc.subject.keywordPlus | RESPONSES | - |
dc.subject.keywordAuthor | Heat stress | - |
dc.subject.keywordAuthor | Proteomics | - |
dc.subject.keywordAuthor | Antarctic microalga | - |
dc.subject.keywordAuthor | Protein expression | - |
dc.subject.keywordAuthor | Antioxidant enzyme | - |
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