Differential protein expression associated with heat stress in Antarctic microalga
- Authors
- Choi, Kwang Man; Lee, Mi Young
- Issue Date
- 20-Sep-2012
- Publisher
- 한국바이오칩학회
- Keywords
- Heat stress; Proteomics; Antarctic microalga; Protein expression; Antioxidant enzyme
- Citation
- BioChip Journal, v.6, no.3, pp 271 - 279
- Pages
- 9
- Journal Title
- BioChip Journal
- Volume
- 6
- Number
- 3
- Start Page
- 271
- End Page
- 279
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/14834
- DOI
- 10.1007/s13206-012-6310-5
- ISSN
- 1976-0280
2092-7843
- Abstract
- We used proteomic approaches to study the survival and defense mechanisms of Antarctic microalga under heat stress. The microalga was cultured for optimal growth at 4 degrees C, the temperature was increased to 15 degrees C, and the resultant differentially expressed proteins induced by heat stress were analyzed by mass spectrometry following two-dimensional gel electrophoresis. Orthologues of 22 proteins showed more than two-fold changes in abundance; of these, 8 proteins were up-regulated, and 14 were down-regulated. In addition, changes in the enzyme activities and isozyme profiles of catalase, aldehyde dehydrogenase, superoxide dismutase, glutathione reductase, and ascorbate peroxidase were investigated using an in-gel activity-staining method. Alterations in protein expression and antioxidant enzyme activity in Antarctic algae may be related to survival and defense mechanisms against elevated temperatures.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
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