Antifouling steroids isolated from red alga epiphyte filamentous bacterium Leucothrix mucor
- Authors
- Cho, Ji Young
- Issue Date
- May-2012
- Publisher
- Blackwell Publishing Inc.
- Keywords
- 17-(1,2,-Dihydroxyl-5-methyl-hexane)-2,3-dihydroxyl-cholest-4-en-6-one; 13-Acetate-17-(1,5-dimethylhexane)-cholest-7-en-3,5,6,15-tetraol; Zoospore inhibition; Diatom; Biofouling bacteria; Nuclear magnetic resonance
- Citation
- Fisheries Science, v.78, no.3, pp 683 - 689
- Pages
- 7
- Journal Title
- Fisheries Science
- Volume
- 78
- Number
- 3
- Start Page
- 683
- End Page
- 689
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15223
- DOI
- 10.1007/s12562-012-0490-8
- ISSN
- 0919-9268
1444-2906
- Abstract
- Active antifouling steroids were isolated from the filamentous bacterium . Bioactivity-guided isolation by silica gel column chromatography and high-performance liquid chromatography generated two compounds. The chemical constituents having the antifouling activities were identified as 17-(1,2-dihydroxyl-5-methyl-hexane)-2,3-dihydroxyl-cholest-4-en-6-one [compound (a)] and 13-acetate-17-(1,5-dimethylhexane)-cholest-7-en-3,5,6,15-tetraol [compound (b)] by interpreting one- and two-dimensional nuclear magnetic resonance and mass spectroscopy data. The two compounds were isolated from 780 mg of crude extract, yielding 12 and 14 mg, respectively. Antifouling activities were determined against a representative soft fouling macroalgae (), a biofouling diatom (), and the bacteria KNP-3 and sp. KNS-8. The two compounds were able to inhibit the spore settlement of zoospores with a low half maximal effective concentration (EC50) (1.2-2.1 mu g/ml) and inhibit diatom growth with an EC50 of 5.2-7.5 mu g/ml. Compound (a) showed activity with minimum inhibitory concentration values of 32-56 mu g/ml and compound (b) showed an activity of 66-90 mu g/ml. Among the two compounds, compound (a) is a new compound, whereas compound (b) was previously reported to have been isolated from a marine invertebrate.
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