Flow Cytometry-Assisted Basophil Activation Test as a Safe Diagnostic Tool for Aspirin/NSAID Hypersenstivity
- Authors
- Kim, Myung Shin; Cho, Young Joo
- Issue Date
- May-2012
- Publisher
- 대한천식알레르기학회
- Keywords
- Flow cytometry; basophil; aspirin; non-steroidal anti-inflammatory drugs; drug hypersensitivity
- Citation
- Allergy, Asthma & Immunology Research, v.4, no.3, pp 137 - 142
- Pages
- 6
- Journal Title
- Allergy, Asthma & Immunology Research
- Volume
- 4
- Number
- 3
- Start Page
- 137
- End Page
- 142
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15231
- DOI
- 10.4168/aair.2012.4.3.137
- ISSN
- 2092-7355
2092-7363
- Abstract
- Purpose: Aspirin and non-steroidal anti-inflammatory drugs (ASA/NSAIDs) are common causes of drug hypersensitivity. An oral provocation test is the only definitive diagnostic test. This study assessed the reliability of a flow cytometry-assisted basophil activation test (FAST) as a safe diagnostic method for ASA/NSAID-induced hypersensitivity, as its high sensitivity and specificity have been demonstrated for many other drugs. Methods: Eighteen patients and 11 controls were enrolled. Using a Flow-CAST kit (R) (Buhlmann Laboratories AG, Schonenbuch, Switzerland), 29 analyses with aspirin, ibuprofen, and diclofenac were performed by flow cytometry to detect double-positive staining of anti-IgE and anti-CD63. The stimulation index was defined as the activated basophil percentage after drug stimulation/basally active basophil percentage. A stimulation index >= 2 and an absolute activated basophil percentage >= 5 were considered positive. Results: Patients with hypersensitivity to ASA/NSAIDs were predominantly female, and the prevalence of atopy was higher in patients than in controls. A sensitivity of 61%, specificity of 91%, positive predictive value of 92%, and negative predictive value of 59% were achieved. Conclusions: FAST is a useful additional method for diagnosis of hypersensitivity reactions to ASA/NSAIDs. Further development is required to increase the sensitivity of the test.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Internal Medicine > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/15231)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.