(3-Chloroacetyl)-indole, a Novel Allosteric AKT Inhibitor, Suppresses Colon Cancer Growth In Vitro and In Vivo
- Authors
- Kim, Dong Joon; Reddy, Kanamata; Kim, Myoung Ok; Li, Yan; Nadas, Janos; Cho, Yong-Yeon; Kim, Jong-Eun; Shim, Jung-Hyun; Song, Nu Ry; Carper, Andria; Lubet, Ronald A.; Bode, Ann M.; Dong, Zigang
- Issue Date
- Nov-2011
- Publisher
- American Association for Cancer Research
- Citation
- Cancer Prevention Research, v.4, no.11, pp 1842 - 1851
- Pages
- 10
- Journal Title
- Cancer Prevention Research
- Volume
- 4
- Number
- 11
- Start Page
- 1842
- End Page
- 1851
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16144
- DOI
- 10.1158/1940-6207.CAPR-11-0158
- ISSN
- 1940-6207
1940-6215
- Abstract
- Indole-3-carbinol (I3C) is produced in Brassica vegetables such as broccoli and cabbage and has been shown to inhibit proliferation and induce apoptosis in various cancer cells, including breast, prostate, colon, and leukemia. However, only high doses of I3C were shown to inhibit cell proliferation (IC50 = 200-300 mu mol/L). Our goal here was to develop a more potent antitumor agent by modifying the structure of I3C. We created I3C derivatives and found that (3-chloroacetyl)-indole (3CAI) more strongly inhibited colon cancer cell growth than I3C. In addition, by screening 85 kinases in a competitive kinase assay, we found that 3CAI was a specific AKT inhibitor. AKT is a serine/threonine kinase that plays a pivotal role in promoting transformation and chemoresistance by inducing proliferation and inhibiting apoptosis. Therefore, AKT is regarded as a critical target for cancer therapy. 3ICA, a derivative of I3C, is a potent and specific AKT inhibitor. This compound showed significant inhibition of AKT in an in vitro kinase assay and suppressed expression of AKT direct downstream targets such as mTOR and GSK3 beta as well as induced growth inhibition and apoptosis in colon cancer cells. In addition, oral administration of this potent AKT inhibitor suppressed cancer cell growth in an in vivo xenograft mouse model. Cancer Prev Res; 4(11); 1842-51. (C) 2011 AACR.
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Collections - College of Medicine > Department of Biochemistry > 1. Journal Articles
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