Identification and Characterization of Adenovirus Early Region 1B-Associated Protein 5 as a Surface Marker on Undifferentiated Human Embryonic Stem Cells
DC Field | Value | Language |
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dc.contributor.author | Choi, Hong Seo | - |
dc.contributor.author | Kim, Won-Tae | - |
dc.contributor.author | Kim, Hana | - |
dc.contributor.author | Kim, Jum-Ji | - |
dc.contributor.author | Ko, Ji-Yun | - |
dc.contributor.author | Lee, Sang-Wang | - |
dc.contributor.author | Jang, Young Joo | - |
dc.contributor.author | Kim, Sang Jick | - |
dc.contributor.author | Lee, Min-Jung | - |
dc.contributor.author | Jung, Han-Sung | - |
dc.contributor.author | Kzhyshkowska, Julia | - |
dc.contributor.author | Um, Soo-Jong | - |
dc.contributor.author | Lee, Mi-Young | - |
dc.contributor.author | Lee, Sang-Hun | - |
dc.contributor.author | Kim, Cheorl-Ho | - |
dc.contributor.author | Ryu, Chun Jeih | - |
dc.date.accessioned | 2021-08-12T05:45:24Z | - |
dc.date.available | 2021-08-12T05:45:24Z | - |
dc.date.issued | 2011-04 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.issn | 1557-8534 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16614 | - |
dc.description.abstract | Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs. | - |
dc.format.extent | 12 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Mary Ann Liebert Inc. | - |
dc.title | Identification and Characterization of Adenovirus Early Region 1B-Associated Protein 5 as a Surface Marker on Undifferentiated Human Embryonic Stem Cells | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1089/scd.2010.0265 | - |
dc.identifier.scopusid | 2-s2.0-79953700592 | - |
dc.identifier.wosid | 000289053000006 | - |
dc.identifier.bibliographicCitation | Stem Cells and Development, v.20, no.4, pp 609 - 620 | - |
dc.citation.title | Stem Cells and Development | - |
dc.citation.volume | 20 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 609 | - |
dc.citation.endPage | 620 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | N | - |
dc.description.journalRegisteredClass | sci | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalResearchArea | Hematology | - |
dc.relation.journalResearchArea | Research & Experimental Medicine | - |
dc.relation.journalResearchArea | Transplantation | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Hematology | - |
dc.relation.journalWebOfScienceCategory | Medicine, Research & Experimental | - |
dc.relation.journalWebOfScienceCategory | Transplantation | - |
dc.subject.keywordPlus | NUCLEAR RIBONUCLEOPROTEIN E1B-AP5 | - |
dc.subject.keywordPlus | SARCOMA EWS PROTEIN | - |
dc.subject.keywordPlus | SELF-RENEWAL | - |
dc.subject.keywordPlus | EXPRESSED NUCLEOLIN | - |
dc.subject.keywordPlus | BINDING-PROTEIN | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | RECEPTOR | - |
dc.subject.keywordPlus | GROWTH | - |
dc.subject.keywordPlus | LINES | - |
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