Identification and Characterization of Adenovirus Early Region 1B-Associated Protein 5 as a Surface Marker on Undifferentiated Human Embryonic Stem Cells
- Authors
- Choi, Hong Seo; Kim, Won-Tae; Kim, Hana; Kim, Jum-Ji; Ko, Ji-Yun; Lee, Sang-Wang; Jang, Young Joo; Kim, Sang Jick; Lee, Min-Jung; Jung, Han-Sung; Kzhyshkowska, Julia; Um, Soo-Jong; Lee, Mi-Young; Lee, Sang-Hun; Kim, Cheorl-Ho; Ryu, Chun Jeih
- Issue Date
- Apr-2011
- Publisher
- Mary Ann Liebert Inc.
- Citation
- Stem Cells and Development, v.20, no.4, pp 609 - 620
- Pages
- 12
- Journal Title
- Stem Cells and Development
- Volume
- 20
- Number
- 4
- Start Page
- 609
- End Page
- 620
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16614
- DOI
- 10.1089/scd.2010.0265
- ISSN
- 1547-3287
1557-8534
- Abstract
- Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.
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Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
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