Effects of pergolide mesylate on transduction efficiency of PEP-1-catalase protein
- Authors
- Sohn, Eun Jeong; Kim, Dae Won; Kim, Young Nam; Kim, So Mi; Lim, Soon Sung; Kang, Tae-Cheon; Kwon, Hyeok Yil; Kim, Duk-Soo; Cho, Sung-Woo; Han, Kyu Hyung; Park, Jinseu; Eum, Won Sik; Hwang, Hyun Sook; Choi, Soo Young
- Issue Date
- 18-Mar-2011
- Publisher
- Academic Press
- Keywords
- Pergolide mesylate; Inflammation; PEP-1-catalase; Protein transduction
- Citation
- Biochemical and Biophysical Research Communications, v.406, no.3, pp 336 - 340
- Pages
- 5
- Journal Title
- Biochemical and Biophysical Research Communications
- Volume
- 406
- Number
- 3
- Start Page
- 336
- End Page
- 340
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16638
- DOI
- 10.1016/j.bbrc.2011.02.038
- ISSN
- 0006-291X
1090-2104
- Abstract
- The low transduction efficiency of various proteins is an obstacle to their therapeutic application. However, protein transduction domains (PTDs) are well-known for a highly effective tool for exogenous protein delivery to cells. We examined the effects of pergolide mesylate (PM) on the transduction of PEP-1-catalase into HaCaT human keratinocytes and mice skin and on the anti-inflammatory activity of PEP-1-catatase against 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation using Western blot and histological analysis. PM enhanced the time- and dose-dependent transduction of PEP-1-catalase into HaCaT cells without affecting the cellular toxicity. In a mouse edema model, PEP-1-catalase inhibited the increased expressions of inflammatory mediators and cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-6 and -1 beta, and tumor necrosis factor-alpha induced by TPA. On the other hand, PM alone failed to exert any significant anti-inflammatory effects. However, the anti-inflammatory effect of co-treatment with PEP-1-catalase and PM was more potent than that of PEP-1-catalase alone. Our results indicate that PM may enhance the delivery of PTDs fusion therapeutic proteins to target cells and tissues and has potential to increase their therapeutic effects of such drugs against various diseases. (C) 2011 Elsevier Inc. All rights reserved.
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Collections - College of Medicine > Department of Anatomy > 1. Journal Articles
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