Proteomic identification of the differentially expressed proteins in human lung epithelial cells by airborne particulate matter
- Authors
- Jeon, Yu Mi; Son, Bu Soon; Lee, Mi Young
- Issue Date
- Jan-2011
- Publisher
- John Wiley & Sons Inc.
- Keywords
- airborne particulate matter; PM10; toxicity; human lung epithelial cells; proteomics
- Citation
- Journal of Applied Toxicology, v.31, no.1, pp 45 - 52
- Pages
- 8
- Journal Title
- Journal of Applied Toxicology
- Volume
- 31
- Number
- 1
- Start Page
- 45
- End Page
- 52
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/16827
- DOI
- 10.1002/jat.1566
- ISSN
- 0260-437X
1099-1263
- Abstract
- Exposure to airborne PM10, particulate matter with a median aerodynamic diameter of less than 10 mu m, is known to be associated with a number of adverse health effects. To gain a better understanding of the cytotoxic mechanism and to develop protein biomarker candidates for PM10-induced toxicity, proteomic analyses were performed in human lung epithelial cells. Two-dimensional gel electrophoresis (2-DE) was followed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) to analyze the proteins differentially expressed by exposure to PM10. Analysis of 2-DE gels revealed more than 1270 protein spots in the cells, of which 36 showed changes of more than 2-fold on exposure to PM10 (up-regulation, n = 6; down-regulation, n = 30). The glycolytic enzyme pyruvate kinase, which also plays a role in tumor metabolism, showed a marked increase in expression, whereas the cytoskeleton-related vinculin and anti-inflammatory annexin 1 showed marked decreases in expression. Copyright (C) 2010 John Wiley & Sons, Ltd.
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- Appears in
Collections - College of Medical Sciences > Department of Medical Biotechnology > 1. Journal Articles
- College of Natural Sciences > Department of Environmental Health Science > 1. Journal Articles
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