Elevated levels of activated and inactivated thrombin-activatable fibrinolysis inhibitor in patients with sepsisElevated levels of activated and inactivated thrombin-activatable fibrinolysis inhibitor in patients with sepsis
- Other Titles
- Elevated levels of activated and inactivated thrombin-activatable fibrinolysis inhibitor in patients with sepsis
- Authors
- 박노진; Jaewoo Song; 안성수
- Issue Date
- 2010
- Publisher
- 대한혈액학회
- Keywords
- Sepsis; TAFI isoforms; TAFIa; TAFIai; Diagnosis
- Citation
- Blood Research, v.45, no.4, pp.264 - 268
- Journal Title
- Blood Research
- Volume
- 45
- Number
- 4
- Start Page
- 264
- End Page
- 268
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/18196
- ISSN
- 2287-979X
- Abstract
- Background In sepsis, large scale inflammatory responses can cause extensive collateral damage to the vasculature, because both coagulation and fibrinolysis are activated unevenly. Thrombin-activatable fibrinolysis inhibitor (TAFI) plays a role in modulating fibrinolysis. Since TAFI can be activated by both thrombin and plasmin, it is thought to be affected in sepsis. Hence, activated and inactivated TAFI (TAFIa/ai) may be used to monitor changes in sepsis.
Methods TAFIa/ai-specific in-house ELISA can detect only the TAFIa/ai form, because the ELISA capture agent is potato tuber carboxypeptidase inhibitor (PTCI), which has selective affinity towards only the TAFIa and TAFIai isoforms. TAFIa/ai levels in plasma from 25 patients with sepsis and 19 healthy volunteers were quantitated with the in-house ELISA.
Results We observed increased TAFIa/ai levels in samples from patients with sepsis (48.7±9.3 ng/mL) than in samples from healthy individuals (10.5±5.9 ng/mL). In contrast, no difference in total TAFI concentration was obtained between sepsis patients and healthy controls. The results suggest that TAFI zymogen was activated and that TAFIa/ai accumulated in sepsis.
Conclusion The detection of TAFIa/ai in plasma could provide a useful and simple diagnostic tool for sepsis. Uneven activation of both coagulation and fibrinolysis in sepsis could be caused by the activation of TAFI zymogen and elevation of TAFIa/ai. TAFIa/ai could be a novel marker to monitor sepsis and other blood-related disturbances.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Clinical Pathology > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.