Increase Effect of Transforming Growth Factor on Eotaxin Production by Normal Cultured Dermal Fibroblasts Stimulated with Interleukin-4: Inhibitory Effect of Suplatast Tosilate on Eotaxin Production
- Authors
- Bae, Sang Jae; Lee, Jeong-Beom; Shimizu, Kazuhiro; Kuwazuka, Yutaka
- Issue Date
- 2010
- Publisher
- Marcel Dekker Inc.
- Keywords
- Fibroblasts; IL-4; TGF-beta; eotaxin; Suplatast tosilate
- Citation
- Immunological Investigations, v.39, no.2, pp 93 - 102
- Pages
- 10
- Journal Title
- Immunological Investigations
- Volume
- 39
- Number
- 2
- Start Page
- 93
- End Page
- 102
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/18580
- DOI
- 10.3109/08820130903496769
- ISSN
- 0882-0139
1532-4311
- Abstract
- Eotaxin plays a central role in the development of allergic disease, including atopic dermatitis, asthma, and nasal allergy. Interleukin (IL)-4 induces eotaxin production in normal human dermal fibroblasts. On the other hands, Transforming growth factor-beta (TGF-beta), a multifunctional regulatory cytokine, affects many biological functions, including fibroblast growth and differentiation and Th2 cytokine regulation. In this study, we investigated the effect of TGF-beta on IL-4-induced eotaxin production by normal human fibroblasts, as well as the effect of suplatast tosilate, an antiallergic drug that selectively inhibits Th2 cytokine production. Dermal fibroblast treatment with IL-4 and TGF-beta for 24 h increased eotaxin production and expression of eotaxin mRNA, as measured by enzyme-linked immunosorbent assay (ELISA) and reverse-transcriptase polymerase chain reaction (RT-PCR), respectively. TGF-beta synergistically up-regulated eotaxin production and eotaxin mRNA expression when stimulated with IL-4. Suplatast tosilate dose-dependently inhibited eotaxin production induced by IL-4 or IL-4 plus TGF-beta. These results suggest that TGF-beta may regulate skin allergic inflammation by up-regulating eotaxin production in dermal fibroblasts. Suplatast tosilate might suppress this inflammation by inhibiting eotaxin production.</.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Medicine > Department of Physiology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/18580)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.