Bifidobacterium longum subsp. infantis YB0411 Inhibits Adipogenesis in 3T3-L1 Pre-adipocytes and Reduces High-Fat-Diet-Induced Obesity in Mice
- Authors
- Rahman, Md Shamim; Kang, Inseok; Lee, Youri; Habib, Md Ahasun; Choi, Byeong Jo; Kang, Jong Soon; Park, Doo-Sang; Kim, Yong-Sik
- Issue Date
- 2-Jun-2021
- Publisher
- American Chemical Society
- Keywords
- probiotics; adipogenesis; anti-obesity; autophagy; AMPK
- Citation
- Journal of Agricultural and Food Chemistry, v.69, no.21, pp 6032 - 6042
- Pages
- 11
- Journal Title
- Journal of Agricultural and Food Chemistry
- Volume
- 69
- Number
- 21
- Start Page
- 6032
- End Page
- 6042
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/18781
- DOI
- 10.1021/acs.jafc.1c01440
- ISSN
- 0021-8561
- Abstract
- Although the health benefits of probiotics have been widely known for decades, there has still been limited use of probiotic bacteria in anti-obesity therapy. Herein, we demonstrated the role of Bifidobacterium longum subsp. infantis YB0411 (YB, which was selected by an in vitro adipogenesis assay) in adipogenic differentiation in 3T3-L1 pre-adipocytes. We observed that YB-treatment effectively reduced triglyceride accumulation and the expression of CCAAT/enhancer-binding protein alpha, beta, and delta (C/EBP alpha, C/EBP beta, and C/EBP delta), peroxisome proliferator-activated receptor gamma (PPAR gamma), fatty acid-binding protein 4 (aP2), and acetylCoA carboxylase (ACC). YB-treatment also reduced the levels of core autophagic markers (p62 and LC3B) in 3T3-L1 preadipocytes. Small-interfering-RNA-mediated knockdown and competitive-chemical-inhibition assays showed that AMP-activated protein kinase (AMPK) commenced the anti-adipogenic effect of YB. In addition, YB supplement markedly reduced body weight and fat accretion in mice with high-fat-diet-induced obesity. Our findings suggest that YB may be used as a potential probiotic candidate to ameliorate obesity.
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