Canonical WNT/beta-Catenin Signaling Activated by WNT9b and RSPO2 Cooperation Regulates Facial Morphogenesis in Miceopen access
- Authors
- Jin, Yong-Ri; Han, Xiang Hua; Nishimori, Katsuhiko; Ben-Avraham, Dan; Oh, Youn Jeong; Shim, Jae-won; Yoon, Jeong Kyo
- Issue Date
- 8-May-2020
- Publisher
- Frontiers Media S.A.
- Keywords
- R-spondin2; Wnt9b; WNT signaling; facial development; cleft lip; cleft palate
- Citation
- Frontiers in Cell and Developmental Biology, v.8
- Journal Title
- Frontiers in Cell and Developmental Biology
- Volume
- 8
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19523
- DOI
- 10.3389/fcell.2020.00264
- ISSN
- 2296-634X
- Abstract
- The R-spondin (RSPO) family of proteins potentiate canonical WNT/beta-catenin signaling and may provide a mechanism to fine-tune the strength of canonical WNT signaling. Although several in vitro studies have clearly demonstrated the potentiation of canonical WNT signaling by RSPOs, whether this potentiation actually occurs in normal development and tissue function in vivo still remains poorly understood. Here, we provide clear evidence of the potentiation of canonical WNT signaling by RSPO during mouse facial development by analyzing compound Wnt9b and Rspo2 gene knockout mice and utilizing ex vivo facial explants. Wnt9b;Rspo2 double mutant mice display facial defects and dysregulated gene expression pattern that are significantly more severe than and different from those of Wnt9b or Rspo2 null mutant mice. Furthermore, we found suggestive evidence that the LGR4/5/6 family of the RSPO receptors may play less critical roles in WNT9b:RSPO2 cooperation. Our results suggest that RSPO-induced cooperation is a key mechanism for fine-tuning canonical WNT/beta-catenin signaling in mouse facial development.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19523)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.