Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs
DC Field | Value | Language |
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dc.contributor.author | Valiulahi, Parvin | - |
dc.contributor.author | Vidyawan, Vincencius | - |
dc.contributor.author | Puspita, Lesly | - |
dc.contributor.author | Oh, Youjin | - |
dc.contributor.author | Juwono, Virginia Blessy | - |
dc.contributor.author | Sittipo, Panida | - |
dc.contributor.author | Friedlander, Gilgi | - |
dc.contributor.author | Yahalomi, Dayana | - |
dc.contributor.author | Sohn, Jong-Woo | - |
dc.contributor.author | Lee, Yun Kyung | - |
dc.contributor.author | Yoon, Jeong Kyo | - |
dc.contributor.author | Shim, Jae-Won | - |
dc.date.accessioned | 2021-10-05T04:42:07Z | - |
dc.date.available | 2021-10-05T04:42:07Z | - |
dc.date.issued | 2021-08-10 | - |
dc.identifier.issn | 2213-6711 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19835 | - |
dc.description.abstract | Serotonin (5-HT) neurons, the major components of the raphe nuclei, arise from ventral hindbrain progenitors. Based on anatomical location and axonal projection, 5-HT neurons are coarsely divided into rostral and caudal groups. Here, we propose a novel strategy to generate hindbrain 5-HT neurons from human pluripotent stem cells (hPSCs), which involves the formation of ventral-type neural progenitor cells and stimulation of the hindbrain 5-HT neural development. A caudalizing agent, retinoid acid, was used to direct the cells into the hindbrain cell fate. Approximately 30%-40% of hPSCs successfully developed into 5-HT-expressing neurons using our protocol, with the majority acquiring a caudal rhombomere identity (r5-8). We further modified our monolayer differentiation system to generate 5-HT neuron-enriched hindbrain-like organoids. We also suggest downstream applications of our 5-HT monolayer and organoid cultures to study neuronal response to gut microbiota. Our methodology could become a powerful tool for future studies related to 5-HT neurotransmission. | - |
dc.format.extent | 15 | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Cell Press | - |
dc.title | Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCs | - |
dc.type | Article | - |
dc.publisher.location | 미국 | - |
dc.identifier.doi | 10.1016/j.stemcr.2021.06.006 | - |
dc.identifier.scopusid | 2-s2.0-85112668972 | - |
dc.identifier.wosid | 000684300500009 | - |
dc.identifier.bibliographicCitation | Stem Cell Reports, v.16, no.8, pp 1938 - 1952 | - |
dc.citation.title | Stem Cell Reports | - |
dc.citation.volume | 16 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1938 | - |
dc.citation.endPage | 1952 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell & Tissue Engineering | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.subject.keywordPlus | RETINOIC ACID SYNTHESIS | - |
dc.subject.keywordPlus | BRAIN-DEVELOPMENT | - |
dc.subject.keywordPlus | HUMAN ES | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MITOCHONDRIAL | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | DYSFUNCTION | - |
dc.subject.keywordPlus | IDENTITY | - |
dc.subject.keywordPlus | MARKER | - |
dc.subject.keywordPlus | PARKIN | - |
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