Generation of caudal-type serotonin neurons and hindbrain-fate organoids from hPSCsopen access
- Authors
- Valiulahi, Parvin; Vidyawan, Vincencius; Puspita, Lesly; Oh, Youjin; Juwono, Virginia Blessy; Sittipo, Panida; Friedlander, Gilgi; Yahalomi, Dayana; Sohn, Jong-Woo; Lee, Yun Kyung; Yoon, Jeong Kyo; Shim, Jae-Won
- Issue Date
- 10-Aug-2021
- Publisher
- Cell Press
- Citation
- Stem Cell Reports, v.16, no.8, pp 1938 - 1952
- Pages
- 15
- Journal Title
- Stem Cell Reports
- Volume
- 16
- Number
- 8
- Start Page
- 1938
- End Page
- 1952
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/19835
- DOI
- 10.1016/j.stemcr.2021.06.006
- ISSN
- 2213-6711
- Abstract
- Serotonin (5-HT) neurons, the major components of the raphe nuclei, arise from ventral hindbrain progenitors. Based on anatomical location and axonal projection, 5-HT neurons are coarsely divided into rostral and caudal groups. Here, we propose a novel strategy to generate hindbrain 5-HT neurons from human pluripotent stem cells (hPSCs), which involves the formation of ventral-type neural progenitor cells and stimulation of the hindbrain 5-HT neural development. A caudalizing agent, retinoid acid, was used to direct the cells into the hindbrain cell fate. Approximately 30%-40% of hPSCs successfully developed into 5-HT-expressing neurons using our protocol, with the majority acquiring a caudal rhombomere identity (r5-8). We further modified our monolayer differentiation system to generate 5-HT neuron-enriched hindbrain-like organoids. We also suggest downstream applications of our 5-HT monolayer and organoid cultures to study neuronal response to gut microbiota. Our methodology could become a powerful tool for future studies related to 5-HT neurotransmission.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
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