기수갈고둥 (Clithon retropictus) 의 Arginine kinase 유전자를 이용한 분자계통학적 연구Molecular Phylogenetic Studies of Clithon retropictus Using Arginine Kinase Gene Sequence
- Other Titles
- Molecular Phylogenetic Studies of Clithon retropictus Using Arginine Kinase Gene Sequence
- Authors
- 홍찬의; 상민규; 송대권; 박지은; 정준양; 김용태; 신현준; 류자미; 황희주; 박소영; 강세원; 이준상; 정기윤; 이용석
- Issue Date
- Sep-2021
- Publisher
- 한국패류학회
- Keywords
- Clithon retropictus; Arginine Kinase; phylogenetic tree
- Citation
- The Korean Journal of Malacology, v.37, no.3, pp 125 - 132
- Pages
- 8
- Journal Title
- The Korean Journal of Malacology
- Volume
- 37
- Number
- 3
- Start Page
- 125
- End Page
- 132
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/21003
- ISSN
- 1225-3480
- Abstract
- Clithon retropictus is a small gastropod belonging to the Neritidae, and inhabits gravel areas in brackish waters. It has been designated as an endangered species as class II, because of its habitat conditions and reckless development of coastlines. In September 2021, C. retropictus registered in NCBI has only 80 nucleotides, 52 proteins, and 74 genes. In this context, we sequenced the C. retropictus cDNA library using the Illumina Hiseq 2500 platform. Arginine Kinase (AK) sequence was obtained by using BLAST program. To investigate the molecular phylogenetic analysis of C. retropictus, a dendrogram was generated using AK gene as a marker. Based on the phylogenetic tree, it is classified into three classes (Cephalopods, Gastropods, and Bivalvia). In addition, analysis of AK 2D structure using 38 species shows that no striking similarity was found. In conclusion, AK gene of C. retropictus consisted of 1,053 bp (351 amino acids) and could be utilized for molecular phylogenetic classification.
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Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
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