Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Mediaopen access
- Authors
- Kim, Byeong-Gon; Choi, Da Yeon; Kim, Min-Gyoung; Jang, An-Soo; Suh, Myung-Whan; Lee, Jun Ho; Oh, Seung Ha; Park, Moo Kyun
- Issue Date
- May-2022
- Publisher
- Frontiers Media S.A.
- Keywords
- diesel exhaust particles; lipopolysaccharides; otitis media; lymphangiogenesis; angiogenesis
- Citation
- Frontiers in cellular and infection microbiology, v.12, no.0, pp 1 - 12
- Pages
- 12
- Journal Title
- Frontiers in cellular and infection microbiology
- Volume
- 12
- Number
- 0
- Start Page
- 1
- End Page
- 12
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/21040
- DOI
- 10.3389/fcimb.2022.824575
- ISSN
- 2235-2988
- Abstract
- Lymphangiogenesis and angiogenesis might have significant involvement in the pathogenesis of otitis media with effusion. This study investigated the effect of diesel exhaust particles (DEP) on inflammation and lymphangiogenesis in a mouse model of acute otitis media (AOM). BALB/c mice were injected with LPS and exposed to 100 mu g/m(3) DEP. The mice were divided into four groups: control (no stimulation), AOM, AOM + DEP, and DEP + AOM.The effects of DEP inhalation pre- and post-DEP induction were estimated based on measurements of the auditory brainstem response, mRNA levels of lymphangiogenesis-related genes and cytokines, and histology of the middle ear. Cell viability of human middle ear epithelial cells decreased in a dose-response manner at 24 and 48 hours post-DEP exposure. DEP alone did not induce AOM. AOM-induced mice with pre- or post-DEP exposure showed thickened middle ear mucosa and increased expression of TNF-alpha and IL1-beta mRNA levels compared to the control group, but increased serum IL-1 beta levels were not found in the AOM + Post DEP. The mRNA expression of TLR4, VEGFA, VEGFAC, and VEGFR3 was increased by pre-AOM DEP exposure.The expression of VEFGA protein was stronger in the AOM + Post DEP group than in any other group. The expression of CD31 and CD45 markers in the mouse middle ear tissue was higher in the Pre DEP + AOM group than in the AOM group. This result implies that pre-exposure to DEP more strongly increases inflammation and lymphangiogenesis in a mouse model of acute otitis media.
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Collections - College of Medicine > Department of Internal Medicine > 1. Journal Articles
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