miR-542-3p Contributes to the HK2-Mediated High Glycolytic Phenotype in Human Glioma Cellsopen access
- Authors
- Kim, Junhyung; Park, Min Woo; Park, Young Joon; Ahn, Ju Won; Sim, Jeong Min; Kim, Suwan; Heo, Jinhyung; Jeong, Ji Hun; 이미혜; Lim, Jaejoon; Moon, Jong-Seok
- Issue Date
- Apr-2021
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- miR-542-3p; HK2; Glycolytic Phenotype; Human Glioma Cells
- Citation
- Genes, v.12, no.5, pp 1 - 14
- Pages
- 14
- Journal Title
- Genes
- Volume
- 12
- Number
- 5
- Start Page
- 1
- End Page
- 14
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/21507
- DOI
- 10.3390/genes12050633
- ISSN
- 2073-4425
- Abstract
- (1) Background: The elevation of glucose metabolism is linked to high-grade gliomas such as glioblastoma multiforme (GBM). The high glycolytic phenotype is associated with cellular proliferation and resistance to treatment with chemotherapeutic agents in GBM. MicroRNA-542-3p (miR-542-3p) has been implicated in several tumors including gliomas. However, the role of miR-542-3p in glucose metabolism in human gliomas remains unclear; (2) Methods: We measured the levels of cellular proliferation in human glioma cells. We measured the glycolytic activity in miR-542-3p knockdown and over-expressed human glioma cells. We measured the levels of miR-542-3p and HK2 in glioma tissues from patients with low- and high-grade gliomas using imaging analysis; (3) Results: We show that knockdown of miR-542-3p significantly suppressed cellular proliferation in human glioma cells. Knockdown of miR-542-3p suppressed HK2-induced glycolytic activity in human glioma cells. Consistently, over-expression of miR-542-3p increased HK2-induced glycolytic activity in human glioma cells. The levels of miR-542-3p and HK2 were significantly elevated in glioma tissues of patients with high-grade gliomas relative to that in low-grade gliomas. The elevation of HK2 levels in patients with high-grade gliomas were positively correlated with the high levels of miR-542-3p in GBM and low-grade gliomas (LGG) based on the datasets from the Cancer Genome Atlas (TCGA) database. Moreover, the high levels of miR-542-3p were associated with poor survival rate in the TCGA database; (4) Conclusions: miR-542-3p contributes to the HK2-mediated high glycolytic phenotype in human glioma cells.
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Collections - Graduate School > Department of Integrated Biomedical Science > 1. Journal Articles
- College of Medicine > Department of Biochemistry > 1. Journal Articles
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