Calcium ionophore-activated platelets induce eosinophil extracellular trap formationopen access
- Authors
- Sim, Myeong Seong; Kim, Hye Jeong; Bae, Ikhyeon; Kim, Chun; Chang, Hun Soo; Choi, Youngwoo; Lee, Dong-Hyun; Park, Hae-Sim; Chung, Il Yup
- Issue Date
- Jul-2023
- Publisher
- Elsevier BV
- Keywords
- Calcium ionophore; Eosinophil; Eosinophil extracellular trap; Platelet; Thrombin
- Citation
- Allergology International, v.72, no.3, pp 466 - 476
- Pages
- 11
- Journal Title
- Allergology International
- Volume
- 72
- Number
- 3
- Start Page
- 466
- End Page
- 476
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/22257
- DOI
- 10.1016/j.alit.2022.12.002
- ISSN
- 1323-8930
1440-1592
- Abstract
- Background: Platelets play a modulatory role in inflammatory response by secreting a vast array of granules and disintegrating into membrane-bound microparticles upon activation. The interplay be-tween eosinophils and platelets is postulated to be implicated in the pathology of allergic airway inflammation. In this study, we investigated whether activated platelets can induce eosinophil extra-cellular trap (EET) formation, a cellular process by which activated eosinophils release net-like DNA fibers.Methods: Platelets were stimulated with the calcium ionophore, A23187, and the platelet agonists, thrombin and adenosine diphosphate (ADP). Platelet cultures were fractionated into conditioned me-dium (CM) and pellet, which were then overlaid on eosinophils to examine EET formation.Results: The CM and pellet from A23187-activated platelets stimulated eosinophils to generate EET, whereas those from thrombin-or ADP-activated platelets failed to induce such generation. The EET-inducing activity of the A23187-activated platelet culture was linearly proportional to the number of activated platelets. Interestingly, while EET formation induced by the direct stimulation of eosinophils with A23187 was NADPH oxidase (NOX)-dependent, EET formation induced by A23187-activated platelets was NOX-independent and significantly inhibited by necroptosis pathway inhibitors.Conclusions: Activated platelets and their products may induce EET formation, thereby potentiating their role in eosinophilic airway inflammation.& COPY; 2022 Japanese Society of Allergology. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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Collections - College of Medicine > Department of Microbiology > 1. Journal Articles
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