Profiling the phyto-constituents of Punica granatum fruits peel extract and accessing its in-vitro antioxidant, anti-diabetic, anti-obesity, and angiotensin-converting enzyme inhibitory properties

Abstract

This context was investigated to assess the in vitro antioxidant, anti-diabetic, anti-obesity, and angiotensin-converting enzyme (ACE) inhibition traits of Punica granatum fruits peel extract. Initially, among various extracts tested, aqueous and ethanolic peel extracts depicted the presence of diverse phytoconstituents. In vitro antioxidative properties of peel extracts were determined using standard methodologies. Results showed that aqueous and ethanolic extracts had IC50 values of 471.7 and 509.16 mu g/mL, respectively in terms of 1,1,diphenyl 2,2,picrylhydrazyl scavenging. Likewise, IC50 values of aqueous and ethanol extract were obtained as 488.76 and 478.47 mu g/mL towards the degradation of hydrogen peroxide. The ethanolic extract exhibited the highest inhibition of a-glucosidase by showing activity of 53.34 2.0 to 15.18 +/- 1.4 U/L in a dose dependent manner (100-1000 mu g/mL). Ethanolic extract was reported as the most active inhibitor of lipase with an IC50 value of 603.50 mu g/mL. Ethanolic extract showed increased inhibition of ACE in a concentration dependent manner (100-1000 mu g/mL) with IC50 value of 519.45 mu g/mL. Fourier transform-infrared spectrum revealed the availability of various functional groups in the ethanolic extract of peel. Gas chromatography-mass spectrometry chromatogram of peel extract illustrated 23 diversified chemical constituents including 1,2,3,4-butanetetrol, Dimethyl sulfone, 9-octadecenamide, and Pentadecanoic acid as predominant compounds. In summary, P. granatum fruits peel extract revealed promising antioxidant, anti-diabetic, anti-obesity, and anti hypertensive properties. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.