Plasmonic Cross-Linking Colorimetric PCR for Simple and Sensitive Nucleic Acid Detection
- Authors
- Jiang, Kunlun; Wu, Jingrui; Kim, Ji-Eun; An, Sujin; Nam, Jwa-Min; Peng, Yung-Kang; Lee, Jung-Hoon
- Issue Date
- May-2023
- Publisher
- American Chemical Society
- Keywords
- Biosensor; Plasmonic photothermal PCR; Photothermal effect; Colorimetric; Nucleic acid detection
- Citation
- Nano Letters, v.23, no.9, pp 3897 - 3903
- Pages
- 7
- Journal Title
- Nano Letters
- Volume
- 23
- Number
- 9
- Start Page
- 3897
- End Page
- 3903
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/24989
- DOI
- 10.1021/acs.nanolett.3c00533
- ISSN
- 1530-6984
1530-6992
- Abstract
- Simple, low-cost, and accurate nucleic acid assay platforms hold great promise for point-of-care (POC) pathogen detection, disease surveillance, and control. Plasmonic photothermal polymerase chain reaction (PPT-PCR) is a powerful and efficient nucleic acid amplification technique, but it lacks a simple and convenient analysis method for POC applications. Herein, we propose a novel plasmonic cross-linking colorimetric PCR (PPTccPCR) assay by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP)-based cross-linking colorimetry. AuNPs form assembled structures with the PMNs in the presence of amplicons and collect in a magnetic field, resulting in color changes to the supernatant. Target DNA with concentrations as low as 5 copies/mu L can be visually detected within 40 min. The achieved limit of detection was 1.8 copies/mu L based on the absorption signals. This simple and sensitive strategy needs no expensive instrumentation and demonstrates high potential for POC detection while enabling further applications in clinical diagnostics.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/24989)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.