A Flexible and Transparent PtNP/SWCNT/PET Electrochemical Sensor for Nonenzymatic Detection of Hydrogen Peroxide Released from Living Cells with Real-Time Monitoring Capabilityopen access
- Authors
- Oh, Da Eun; Lee, Chang-Seuk; Kim, Tae Wan; Jeon, Seob; Kim, Tae Hyun
- Issue Date
- Jul-2023
- Publisher
- MDPI
- Keywords
- electrochemical sensing; flexible sensor; hydrogen peroxide (H2O2); platinum nanoparticles; single-walled carbon nanotube (SWCNT) network
- Citation
- BIOSENSORS-BASEL, v.13, no.7
- Journal Title
- BIOSENSORS-BASEL
- Volume
- 13
- Number
- 7
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25387
- DOI
- 10.3390/bios13070704
- ISSN
- 2079-6374
2079-6374
- Abstract
- We developed a transparent and flexible electrochemical sensor using a platform based on a network of single-walled carbon nanotubes (SWCNTs) for the non-enzymatic detection of hydrogen peroxide (H2O2) released from living cells. We decorated the SWCNT network on a poly(ethylene terephthalate) (PET) substrate with platinum nanoparticles (PtNPs) using a potentiodynamic method. The PtNP/SWCNT/PET sensor synergized the advantages of a flexible PET substrate, a conducting SWCNT network, and a catalytic PtNP and demonstrated good biocompatibility and flexibility, enabling cell adhesion. The PtNP/SWCNT/PET-based sensor demonstrated enhanced electrocatalytic activity towards H2O2, as well as excellent selectivity, stability, and reproducibility. The sensor exhibited a wide dynamic range of 500 nM to 1 M, with a low detection limit of 228 nM. Furthermore, the PtNP/SWCNT/PET sensor remained operationally stable, even after bending at various angles (15 & DEG;, 30 & DEG;, 60 & DEG;, and 90 & DEG;), with no noticeable loss of current signal. These outstanding characteristics enabled the PtNP/SWCNT/PET sensor to be practically applied for the direct culture of HeLa cells and the real-time monitoring of H2O2 release by the HeLa cells under drug stimulation.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Chemistry > 1. Journal Articles
- College of Medicine > Department of Obstetrics and Gynecology > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25387)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.