A Novel Interaction between MFN2/Marf and MARK4/PAR-1 Is Implicated in Synaptic Defects and Mitochondrial Dysfunctionopen access
- Authors
- Cheon, Yeongmi; Yoon, Sunggyu; Lee, Jae-Hyuk; Kim, Kiyoung; Kim, Hyung-Jun; Hong, Sung Wook; Yun, Ye -Rang; Shim, Jiwon; Kim, Sung-Hak; Lu, Bingwei; Lee, Mihye; Lee, Seongsoo
- Issue Date
- Aug-2023
- Publisher
- SOC NEUROSCIENCE
- Keywords
- Drosophila melanogaster; MARK4/PAR-1; MFN2/Marf; mitochondrial dynamics; neurodegenerative disease
- Citation
- ENEURO, v.10, no.8
- Journal Title
- ENEURO
- Volume
- 10
- Number
- 8
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25478
- DOI
- 10.1523/ENEURO.0409-22.2023
- ISSN
- 2373-2822
- Abstract
- (Marf), plays a role in mitochondrial fusion, mutations of which are implicated in age-related human diseases, including several neurodegenerative disorders. However, the regulation of MFN2/Marf-mediated mitochondrial fusion, as well as the pathologic mechanism of neurodegeneration, is not clearly understood. Here, we identi-fied a novel interaction between MFN2/Marf and microtubule affinity-regulating kinase 4 (MARK4)/PAR-1. In the Drosophila larval neuromuscular junction, muscle-specific overexpression of MFN2/Marf decreased the number of synaptic boutons, and the loss of MARK4/PAR-1 alleviated the synaptic defects of MFN2/Marf overexpression. Downregulation of MARK4/PAR-1 rescued the mitochondrial hyperfusion phenotype caused by MFN2/Marf overexpression in the Drosophila muscles as well as in the cultured cells. In addition, knock-down of MARK4/PAR-1 rescued the respiratory dysfunction of mitochondria induced by MFN2/Marf overex-pression in mammalian cells. Together, our results indicate that the interaction between MFN2/Marf and MARK4/PAR-1 is fine-tuned to maintain synaptic integrity and mitochondrial homeostasis, and its dysregula-tion may be implicated in neurologic pathogenesis.Significance StatementWe identified a novel interaction between MFN2/Marf and kinase MARK4/PAR-1 in Drosophila and mamma-lian cells. The MFN2/Marf and MARK4/PAR-1 interaction was critical for maintaining the synaptic structure of neuromuscular junctions in Drosophila. In addition, we found that concomitant knockdown of MARK4/ PAR-1 could rescue the mitochondrial hyperfusion and aberrant respiratory function caused by MFN2/Marf overexpression. Our study provides new insights into the link between mitochondrial defects and neurode-generation, which makes a significant contribution to the understanding of neurologic pathogenesis and therapeutic development.
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Collections - College of Medicine > Department of Biochemistry > 1. Journal Articles
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