액체크로마토그래피-질량분석법 기반 전혈 내 면억억제제 약물농도 정량검사에 접목한 Andrew+ 검체 로봇 전처리방법 평가Evaluation of a Robotic Liquid Handler, Andrew+, for Sample Preparation for LC-MS/MS-Based Quantitation of Immunosuppressants in Whole Blood
- Other Titles
- Evaluation of a Robotic Liquid Handler, Andrew+, for Sample Preparation for LC-MS/MS-Based Quantitation of Immunosuppressants in Whole Blood
- Authors
- 채효진; 김혜정; 김정은; 이정중; 조성은; 김솔잎; 이경훈; 이상국; 이용화; 박형두; 박승만; 송상훈; 전사일; 송정한; 대한임상화학회 임상질량분석연구회
- Issue Date
- Jul-2023
- Publisher
- 대한진단검사의학회
- Keywords
- Automated liquid handling; Immunosuppressive drug; Liquid chromatography/tandem mass spectrometry; Sample preparation
- Citation
- Laboratory Medicine Online, v.13, no.3, pp 199 - 204
- Pages
- 6
- Journal Title
- Laboratory Medicine Online
- Volume
- 13
- Number
- 3
- Start Page
- 199
- End Page
- 204
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/25743
- DOI
- 10.47429/lmo.2023.13.3.199
- Abstract
- Background: Liquid chromatography/tandem mass spectrometry (LC-MS/MS)-based assays are increasingly being used for therapeutic drug monitoring (TDM) of immunosuppressive drugs (ISDs). However, many laboratories that employ LC-MS/MS assays for TDM of ISDs use manual sample preparation steps.
Methods: We implemented a semi-automated sample preparation method based on protein precipitation, using an Andrew+ liquid handler, integrated with an LC-MS/MS-based TDM assay for four ISDs (tacrolimus, sirolimus, everolimus, and cyclosporine A). The feasibility and analytical performance were analyzed and compared with those of the manual sample preparation method using 163 clinical samples.
Results: The total execution time of Andrew+-based sample preparation method was 55 minutes (including off-line centrifugation) for 48 samples. However, the hands-on time was significantly reduced than that of the manual sample preparation method. The average repeatability and within-laboratory precision of the assay were acceptable (CV ≤10%), and the results of manual and Andrew+-based sample preparation methods were equivalent.
Conclusions: The sample preparation method using the Andrew+ liquid handler was successfully integrated with LC-MS/MS-based TDM assays for ISDs. Sample preparation using Andrew+ was reproducible, standardized, and enabled excellent utilization of technical staff time. Although further optimization for full automation is still pursued, this study indicates the potential of integrating liquid handers with LC-MS/MS-based assays in the clinical setting.
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