Melatonin Suppresses Renal Cortical Fibrosis by Inhibiting Cytoskeleton Reorganization and Mitochondrial Dysfunction through Regulation of miR-4516open access
- Authors
- Yoon, Yeo Min; Go, Gyeongyun; Yun, Chul Won; Lim, Ji Ho; Lee, Jun Hee; Lee, Sang Hun
- Issue Date
- Aug-2020
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- melatonin; miR-4516; renal cortical fibrosis; TH1 cells; cytoskeleton reorganization
- Citation
- International Journal of Molecular Sciences, v.21, no.15
- Journal Title
- International Journal of Molecular Sciences
- Volume
- 21
- Number
- 15
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2596
- DOI
- 10.3390/ijms21155323
- ISSN
- 1661-6596
1422-0067
- Abstract
- Renal fibrosis, a major risk factor for kidney failure, can lead to chronic kidney disease (CKD) and is caused by cytoskeleton reorganization and mitochondrial dysfunction. In this study, we investigated the potential of melatonin treatment to reduce renal fibrosis by recovering the cytoskeleton reorganization and mitochondrial dysfunction. We found that miR-4516 expression was downregulated in the renal cortex of CKD mice andP-cresol-treated TH1 cells. Decreased miR-4516 expression stimulated cytoskeleton reorganization and mitochondrial dysfunction, and induced renal fibrosis. Melatonin treatment suppressed fibrosis by inhibiting cytoskeleton reorganization and restoring mitochondrial function via increased miR-4516 expression. More specifically, melatonin treatment increased miR-4516 expression while decreasing ITGA9 expression, thereby inhibiting cytoskeleton reorganization. In addition, increased expression of miR-4516 by melatonin treatment reduced ROS formation and restored mitochondrial function. These findings suggest that melatonin may be a promising treatment for patients with CKD having renal fibrosis. Moreover, regulation of miR-4516 expression may be a novel strategy for the treatment of renal fibrosis.
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Collections - College of Medicine > Department of Biochemistry > 1. Journal Articles
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