In silico identification and expression analysis of superoxide dismutases in <i>Tenebrio molitor</i>
- Authors
- Jang, Ho Am; Shin, Hyeonjun; Lee, Seo Jin; Ku, Sung Min; Kim, Jae Hui; Kang, Dong Woo; Choi, So Yeon; Jung, Sang Mok; Shin, Hyun Woung; Lee, Yong Seok; Han, Yeon Soo; Jo, Yong Hun
- Issue Date
- May-2024
- Publisher
- SPRINGER
- Keywords
- Tenebrio molitor; Superoxide dismutase; Developmental stage; Tissue specific; mRNA level
- Citation
- GENES & GENOMICS
- Journal Title
- GENES & GENOMICS
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26241
- DOI
- 10.1007/s13258-024-01518-6
- ISSN
- 1976-9571
2092-9293
- Abstract
- Background Insects encounter various environmental stresses, in response to which they generate reactive oxygen species (ROS). Superoxide dismutase (SOD) is an antioxidant metalloenzyme that scavenges superoxide radicals to prevent oxidative damage. Objective To investigate expressions of SODs under oxidative stress in Tenebrio molitor. Methods Here, we investigated the transcriptional expression of SODs by pesticide and heavy metals in Tenebrio moltior. First, we searched an RNA-Seq database for T. molitor SOD (TmSOD) genes and identified two SOD isoforms (TmSOD1-iso1 and iso2). We examined their activities under developmental stage, tissue-specific, and various types (pesticide and heavy metal) of oxidative stress by using qPCR. Results Our results revealed two novel forms of TmSODs. These TmSODs had a copper/zinc superoxide dismutase domain, active site, Cu2+ binding site, Zn2+ binding site, E-class dimer interface, and P-class dimer interface. TmSODs (TmSOD1-iso1 and iso2) were expressed in diverse developmental phases and tissues. Pesticides and heavy metals caused an upregulation of these TmSODs. Conclusion Our findings suggest that the two TmSODs have different functions in T. molitor, providing insights into the detoxification ability of T. molitor.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - College of Natural Sciences > Department of Biology > 1. Journal Articles
- College of Natural Sciences > Institute of Basic Sciences Research > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26241)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.