Anti-osteoporotic effects of enzymatic hydrolysates of mackerel <i>Scomber japonicus</i> byproduct in MC3T3-E1 cells and ovariectomized rat model
- Authors
- Im, Seung Tae; Kim, Minji; Kim, Wook Chul; Lee, Yun-Su; Lee, Seung-Hong
- Issue Date
- May-2024
- Publisher
- SPRINGER JAPAN KK
- Keywords
- Scomber japonicus byproduct; Protein hydrolysate; Ovariectomized rat; Antiosteoporosis; Functional food
- Citation
- FISHERIES SCIENCE
- Journal Title
- FISHERIES SCIENCE
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26283
- DOI
- 10.1007/s12562-024-01789-8
- ISSN
- 0919-9268
1444-2906
- Abstract
- Recently, interest in fish byproduct-derived bioactive components as potential ingredients for treating and preventing osteoporosis is increasing. In this study, we investigated the antiosteoporotic effects of mackerel Scomber japonicus extracts in MC3T3-E1 preosteoblasts in vitro and ovariectomized rats in vivo. Scomber japonicus neutrase-assisted protein hydrolysate (SJNH) treatment enhanced alkaline phosphatase (ALP) activity and osteoprotegerin (OPG) level, and attenuated receptor activator of nuclear factor kappa B ligand (RANKL) level in MC3T3-E1 cells. In an osteoporotic animal model, ovariectomized (OVX) rats were administered SJNH (200 mg/kg/day) for 8 weeks. SJNH prevented OVX-induced bone mineral density (BMD) loss and restored bone structural compartment loss based on micro-computed tomography (CT). In addition, SJNH administration normalized bone remodeling by regulating bone regeneration and resorption-related proteins such as ALP, osteocalcin (OC), and tartrate-resistant acid phosphatase 5b (TRACP-5b) as well as calcium contents in serum. Taken together, our findings suggest that SJNH can promote antiosteoporotic effects both in vitro and in vivo. Thus, SJNH might be a potent therapeutic agent or functional food ingredient for preventing osteoporosis.
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Collections - College of Medical Sciences > Department of Pharmaceutical Engineering > 1. Journal Articles
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