Investigation of Physical Characteristics and In Vitro Anti-Inflammatory Effects of Fucoidan from <i>Padina arborescens</i>: A Comprehensive Assessment against Lipopolysaccharide-Induced Inflammationopen access
- Authors
- Lee, Hyo-Geun; Liyanage, N. M.; Yang, Fengqi; Kim, Young-Sang; Lee, Seung-Hong; Ko, Seok-Chun; Yang, Hye-Won; Jeon, You-Jin
- Issue Date
- Mar-2024
- Publisher
- MDPI
- Keywords
- Padina arborescens; fucoidan; anti-inflammatory activity; structural characteristics; functional food
- Citation
- MARINE DRUGS, v.22, no.3
- Journal Title
- MARINE DRUGS
- Volume
- 22
- Number
- 3
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26396
- DOI
- 10.3390/md22030109
- ISSN
- 1660-3397
- Abstract
- A biocompatible, heterogeneous, fucose-rich, sulfated polysaccharide (fucoidan) is biosynthesized in brown seaweed. In this study, fucoidan was isolated from Padina arborescens (PAC) using celluclast-assisted extraction, purified, and evaluated for its anti-inflammatory potential in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Structural analyses were performed using Fourier transform infrared (FTIR) and scanning electron microscopy. Among the purified fucoidans, fucoidan fraction 5 (F5) exhibited strong inhibitory activity against LPS-induced nitric oxide (NO) production and pro-inflammatory cytokine generation through the regulation of iNOS/COX-2, MAPK, and NF-kappa B signaling in LPS-induced RAW 264.7 cells. Determination of the structural characteristics indicated that purified F5 exhibited characteristics similar to those of commercial fucoidan. In addition, further analyses suggested that F5 inhibits LPS-induced toxicity, cell death, and NO generation in zebrafish models. Taken together, these findings imply that P. arborescens fucoidans have exceptional anti-inflammatory action, both in vitro and in vivo, and that they may have prospective uses in the functional food sector.
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Collections - College of Medical Sciences > Department of Pharmaceutical Engineering > 1. Journal Articles
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