염주알다슬기 (Koreanomelania nodifila) 의 Arginine Kinase 유전자를 이용한 분자계통학적 분석Molecular Phylogenetic Analysis of Koreanomelania nodifila using Arginine Kinase gene
- Other Titles
- Molecular Phylogenetic Analysis of Koreanomelania nodifila using Arginine Kinase gene
- Authors
- 이혁; 상민규; 송대권; 박지은; 정준양; 홍찬의; 김용태; 신현준; 장이선; 황희주; 강세원; 박소영; 박홍석; 이준상; 한연수; 이용석
- Issue Date
- Dec-2023
- Publisher
- 한국패류학회
- Keywords
- Koreanomelania nodifila; Arginine Kinase; phylogenetic tree
- Citation
- The Korean Journal of Malacology, v.39, no.4, pp 199 - 205
- Pages
- 7
- Journal Title
- The Korean Journal of Malacology
- Volume
- 39
- Number
- 4
- Start Page
- 199
- End Page
- 205
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/26565
- ISSN
- 1225-3480
- Abstract
- Arginine kinases (AK) are known to be widely distributed in various invertebrates. It has an essential role in cellular energy metabolism. It regulates energy regulation and homeostasis in response to external stress. AK gene is used as a marker in invertebrate phylogenetic analysis. In this study, we verified whether AK can be used in molecular phylogenetic analysis. We sequenced Koreanomelania nodifila cDNA library using Illumina Hiseq 2000. The AK gene of K. nodifila was sequenced using the BLASTx program and PANM DB (v5.1). AK gene was used as a marker to molecular phylogenetic using the MEGA 11 maximum likelihood method. To analyze the 2D structure of the AK gene, the 2D structure was predicted using PSIPRED (v4.0). As a result, the AK gene ORF of K. nodifila is a total of 352 amino acids and AK gene is considered to be highly useful for molecular phylogenetic analysis of Mollusca.
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Collections - College of Medicine > Department of Neurosurgery > 1. Journal Articles
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