염주알다슬기 (Koreanomelania nodifila) 의 Arginine Kinase 유전자를 이용한 분자계통학적 분석

Abstract

Arginine kinases (AK) are known to be widely distributed in various invertebrates. It has an essential role in cellular energy metabolism. It regulates energy regulation and homeostasis in response to external stress. AK gene is used as a marker in invertebrate phylogenetic analysis. In this study, we verified whether AK can be used in molecular phylogenetic analysis. We sequenced Koreanomelania nodifila cDNA library using Illumina Hiseq 2000. The AK gene of K. nodifila was sequenced using the BLASTx program and PANM DB (v5.1). AK gene was used as a marker to molecular phylogenetic using the MEGA 11 maximum likelihood method. To analyze the 2D structure of the AK gene, the 2D structure was predicted using PSIPRED (v4.0). As a result, the AK gene ORF of K. nodifila is a total of 352 amino acids and AK gene is considered to be highly useful for molecular phylogenetic analysis of Mollusca.