Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages
DC Field | Value | Language |
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dc.contributor.author | Kim, Nam-Hun | - |
dc.contributor.author | Kim, Hong-Ki | - |
dc.contributor.author | Lee, Ji-Hak | - |
dc.contributor.author | Jo, Seung-Il | - |
dc.contributor.author | Won, Hye-Min | - |
dc.contributor.author | Lee, Gyeong-Seok | - |
dc.contributor.author | Lee, Hyoun-Su | - |
dc.contributor.author | Nam, Kung-Woo | - |
dc.contributor.author | Kim, Wan-Jong | - |
dc.contributor.author | Han, Man-Deuk | - |
dc.date.accessioned | 2021-08-11T08:34:20Z | - |
dc.date.available | 2021-08-11T08:34:20Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2675 | - |
dc.description.abstract | The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effects on reactive oxygen species (ROS) and NLRP3 ATPase activity were assessed. The expression levels of NLRP3, caspase-1, and pro-inflammatory cytokines (IL-1 beta, IL-18) and cytotoxicity of juglone in J774.1 cells were also determined. Juglone was non-toxic in J774.1 cells when used at 10 mu M (p < 0.01). Juglone treatment inhibited the production of ROS and NO. The levels of NLRP3 and cleaved caspase-1, as well as the secretion of IL-1 beta and IL-18, were decreased by treatment with juglone in a concentration-dependent manner. Juglone also inhibited the ATPase activities of NLRP3 in LPS/ATP-stimulated J774.1 macrophages. Our results suggested that juglone could inhibit inflammatory cytokine production and NLRP3 inflammasome activation in macrophages, and should be considered as a therapeutic strategy for inflammation-related diseases. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | - |
dc.title | Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophages | - |
dc.type | Article | - |
dc.publisher.location | 스위스 | - |
dc.identifier.doi | 10.3390/molecules25133104 | - |
dc.identifier.scopusid | 2-s2.0-85087837500 | - |
dc.identifier.wosid | 000550270600001 | - |
dc.identifier.bibliographicCitation | Molecules, v.25, no.13 | - |
dc.citation.title | Molecules | - |
dc.citation.volume | 25 | - |
dc.citation.number | 13 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.subject.keywordPlus | PATTERN-RECOGNITION | - |
dc.subject.keywordPlus | IL-1-BETA | - |
dc.subject.keywordPlus | INHIBITOR | - |
dc.subject.keywordPlus | MELANOMA | - |
dc.subject.keywordPlus | ATP | - |
dc.subject.keywordAuthor | juglone | - |
dc.subject.keywordAuthor | NLRP3 inflammasome | - |
dc.subject.keywordAuthor | caspase-1 | - |
dc.subject.keywordAuthor | IL-1 beta | - |
dc.subject.keywordAuthor | IL-18 | - |
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