Juglone Suppresses LPS-induced Inflammatory Responses and NLRP3 Activation in Macrophagesopen access
- Authors
- Kim, Nam-Hun; Kim, Hong-Ki; Lee, Ji-Hak; Jo, Seung-Il; Won, Hye-Min; Lee, Gyeong-Seok; Lee, Hyoun-Su; Nam, Kung-Woo; Kim, Wan-Jong; Han, Man-Deuk
- Issue Date
- Jul-2020
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- juglone; NLRP3 inflammasome; caspase-1; IL-1 beta; IL-18
- Citation
- Molecules, v.25, no.13
- Journal Title
- Molecules
- Volume
- 25
- Number
- 13
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2675
- DOI
- 10.3390/molecules25133104
- ISSN
- 1420-3049
- Abstract
- The NLRP3 (NACHT, LRR and PYD domains-containing protein 3) inflammasome has been implicated in a variety of diseases, including atherosclerosis, neurodegenerative diseases, and infectious diseases. Thus, inhibitors of NLRP3 inflammasome have emerged as promising approaches to treat inflammation-related diseases. The aim of this study was to explore the effects of juglone (5-hydroxyl-1,4-naphthoquinone) on NLRP3 inflammasome activation. The inhibitory effects of juglone on nitric oxide (NO) production were assessed in lipopolysaccharide (LPS)-stimulated J774.1 cells by Griess assay, while its effects on reactive oxygen species (ROS) and NLRP3 ATPase activity were assessed. The expression levels of NLRP3, caspase-1, and pro-inflammatory cytokines (IL-1 beta, IL-18) and cytotoxicity of juglone in J774.1 cells were also determined. Juglone was non-toxic in J774.1 cells when used at 10 mu M (p < 0.01). Juglone treatment inhibited the production of ROS and NO. The levels of NLRP3 and cleaved caspase-1, as well as the secretion of IL-1 beta and IL-18, were decreased by treatment with juglone in a concentration-dependent manner. Juglone also inhibited the ATPase activities of NLRP3 in LPS/ATP-stimulated J774.1 macrophages. Our results suggested that juglone could inhibit inflammatory cytokine production and NLRP3 inflammasome activation in macrophages, and should be considered as a therapeutic strategy for inflammation-related diseases.
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Collections - College of Natural Sciences > Department of Life Science and Biotechnology > 1. Journal Articles
- College of Natural Sciences > Department of Biology > 1. Journal Articles
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