Molecular and Histologic Evidence of Novel Erectile Dysfunction Rat Model as an Aging Atherosclerosis Model: A Preliminary Studyopen access
- Authors
- Kim, Jae Heon; Shim, Ji Sung; Kim, Jong Wook; Doo, Seung Whan; Bae, Jae Hyun; Lee, Ju Han; Song, Yun Seob; Kim, Je Jong; Moon, Du Geon
- Issue Date
- Jul-2020
- Publisher
- 대한남성과학회
- Keywords
- Atherosclerosis; Erectile dysfunction; Phosphodiesterase 5 Inhibitors; Vasculogenic impotence
- Citation
- The World Journal of Men's Health, v.38, no.3, pp 345 - 352
- Pages
- 8
- Journal Title
- The World Journal of Men's Health
- Volume
- 38
- Number
- 3
- Start Page
- 345
- End Page
- 352
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2689
- DOI
- 10.5534/wjmh.190031
- ISSN
- 2287-4208
2287-4690
- Abstract
- Purpose: To validate a novel arteriogenic erectile dysfunction (ED) model with atherosclerosis (AS) based on molecular and histologic evidence induced by chronic pelvic ischemia (CPI) and determine effect of phosphodiesterase-5 inhibitor treatment. Materials and Methods: Twenty 16-week-old male Sprague-Dawley rats were divided into three experimental groups (Group I, untreated sham-operated rats with regular diet; Group II, CPI with cholesterol diet; Group III, CPI model with cholesterol diet and mirodenafil). Erectile function was accessed using maximum intracavernous pressure (ICP) and ICP/mean arterial pressure (MAP). Molecular changes were examined by western blot analysis using hypoxia inducible factor 1-alpha (HIF-1 alpha), endothelial nitric oxide synthase (eNOS), and transforming growth factor beta-1 (TGF-beta 1) antibodies. Collagen change was evaluated by Masson's trichrome staining. Results: In vivo measurements of ICP and ICP/MAP in Group II were significantly lower than those in Group I (p<0.01). Smooth muscle/collagen ratio in Group II was significantly lower than that in Group I (p<0.05). After treatment with mirodenafil for four weeks, Group III showed significantly higher levels of ICP and ICP/MAP than Group II (p<0.05). Western blot analysis showed that HIF-1 alpha and TGF-beta 1 levels were significantly higher in Group II whereas eNOS levels were significantly lower in Group II than those in Group I or III. Conclusions: A novel arteriogenic ED with AS model is successfully induced by CPI and validated based on molecular and histologic evidences.
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Collections - College of Medicine > Department of Urology > 1. Journal Articles
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