Cuprizone Affects Hypothermia-Induced Neuroprotection and Enhanced Neuroblast Differentiation in the Gerbil Hippocampus after Ischemiaopen access
- Authors
- Kim, Woosuk; Hahn, Kyu Ri; Jung, Hyo Young; Kwon, Hyun Jung; Nam, Sung Min; Kim, Tae Hyeong; Kim, Jong Whi; Yoo, Dae Young; Kim, Dae Won; Choi, Jung Hoon; Yoon, Yeo Sung; Hwang, In Koo
- Issue Date
- Jun-2020
- Publisher
- Multidisciplinary Digital Publishing Institute (MDPI)
- Keywords
- cuprizone; ischemia; hippocampus; neurogenesis; hypothermia
- Citation
- Cells, v.9, no.6
- Journal Title
- Cells
- Volume
- 9
- Number
- 6
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/2780
- DOI
- 10.3390/cells9061438
- ISSN
- 2073-4409
- Abstract
- In the present study, we investigated the effects of cuprizone on cell death, glial activation, and neuronal plasticity induced by hypothermia after ischemia in gerbils. Food was supplemented with cuprizone at 0.2% ad libitum for eight weeks. At six weeks after diet feeing, gerbils received transient forebrain ischemia with or without hypothermic preconditioning. Cuprizone treatment for 8 weeks increased the number of astrocytes, microglia, and pro-inflammatory cytokine levels in the hippocampus. In addition, cuprizone treatment significantly decreased the number of proliferating cells and neuroblasts in the dentate gyrus. Brain ischemia caused cell death, disruption of myelin basic proteins, and reactive gliosis in CA1. In addition, ischemia significantly increased pro-inflammatory cytokines and the number of proliferating cells and differentiating neuroblasts in the dentate gyrus. In contrast, hypothermic conditioning attenuated these changes in CA1 and the dentate gyrus. However, cuprizone treatment decreased cell survival induced by hypothermic preconditioning after ischemia and increased the number of reactive microglia and astrocytes in CA1 as well as that of macrophages in the subcallosal zone. These changes occurred because the protective effect of hypothermia in ischemic damage was disrupted by cuprizone administration. Furthermore, cuprizone decreased ischemia-induced proliferating cells and neuroblasts in the dentate gyrus.
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Collections - College of Medicine > Department of Anatomy > 1. Journal Articles
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