Antioxidant and anti-melanogenic activities of ultrasonic extract from Stichopus japonicusopen access
- Authors
- Ding, Yuling; Jiratchayamaethasakul, Chanipa; Kim, Junseong; Kim, Eun-A; Heo, Soo-Jin; Lee, Seung-Hong
- Issue Date
- Jan-2020
- Publisher
- Elsevier BV
- Keywords
- Red sea cucumber (Stichopus japonicus); Ultrasonicextract; Antioxidant activity; Anti-melanogenesis; Cosmeceuticals
- Citation
- Asian Pacific Journal of Tropical Biomedicine, v.10, no.1, pp 33 - 41
- Pages
- 9
- Journal Title
- Asian Pacific Journal of Tropical Biomedicine
- Volume
- 10
- Number
- 1
- Start Page
- 33
- End Page
- 41
- URI
- https://scholarworks.bwise.kr/sch/handle/2021.sw.sch/3258
- DOI
- 10.4103/2221-1691.273092
- ISSN
- 2221-1691
2588-9222
- Abstract
- Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island.Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes (HaCaT) to determine its radical scavenging activity and protective effects against 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress. Anti-melanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by alpha-melanocyte-stimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins (TRP-1, and TRP-2) were examined.Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50: (0.924 +/- 0.035) and (0.327 +/- 0.006) mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPH- induced HaCaT cells, with no cytotoxicity (12.5-400 mu g/mL). The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50: (2.750 +/- 0.006) mg/mL]. On alpha-melanocyte-stimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber (25-200 mu g/mL) significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2.Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry.
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